This study focuses on an automated Substrate Pulse Batch (SPB) technique used for the mass cultivation of bacteria adapted to the degradation of a mixture composed of toluene, ethylbenzene, o-, m- and p-xylenes (TEX). A small-scale prototype reactor was designed. A computer-based monitoring program was also developed. The key parameters to be monitored were handled by LabVIEW including, temperature, pH, dissolved oxygen and turbidity. Other parameters, such as biomass, ammonium or residual substrate concentrations needed offline measurements.
SPB technique has been successfully tested experimentally on TEX. The dynamic behavior of the mixed bacterial population was observed under different operational conditions. Average productivity and yield values obtained were 0.45 kgDW m-3 d-1 and 0.59 gDW gC-1, respectively. These data come up to the industrial specifications and confirm the benefits of such an improved technology. Adapted microorganisms obtained in this way can play a major role in the removal of hydrocarbons from industrial off-gases, degradation of substitute organic solvents, reduction in odors from wastewater plants and the food industry or even the removal of pollutant mixtures containing chlorinated solvents.
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