Assessment of p38? in peripheral blood mononuclear cells (PBMC): a potential blood protein marker of head and neck squamous cell carcinoma

• Autores: K. Gill, R. Kumar, B. Kalyan Mohanti, S. Dey
• Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 15, Nº. 11, 2013, págs. 969-973
• Idioma: inglés
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• Resumen

  • Purpose Solid tumors involve an inflammatory microenvironment portrayed by immune cells playing role in cancer progression via inflammatory p38? mitogen-activated protein kinase (MAPK) molecule that produces pro-inflammatory cytokines-TNF?, IL1? and IL6. This study quantified and compared the expression of p38? in peripheral blood mononuclear cells (PBMCs) of HNSCC patients with the healthy subjects.

    Methods The PBMC were isolated from the 35 control and 83 HNSCC patients. The expression of p38? in PBMCs was assessed using surface plasmon resonance (SPR), ELISA and western blot analysis.

    Results p38? levels were found to be over-expressed in HNSCC patients 0.98 ng/?l (95 % CI 0.95�1.02) as compared to controls 0.46 ng/?l (95 % CI 0.42�0.50) (p < 0.0001).

    Conclusion p38? is over-expressed in PBMCs of HNSCC patients and may play a role in the progression of cancer. This research may translate a protein marker for HNSCC to clinical oncologist for therapeutic intervention and use as a predictive marker.