Succinate cytochrome c reductase in schistosomiasis: in vitro inhibition by some schistosomicidal drugs

• Autores: Mahmoud Balbaa, Nihad M. Abdel Moneam, Mohamed El-Kersh, Heba Omran, Kamal Kandeel
• Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 66, Nº. 4, 2010, págs. 291-299
• Idioma: inglés
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• Resumen

  • Enzymes in mitochondria play an important role in biological oxidation and energy production. To understand the effect of schistosomiasis on these important processes, succinate cytochrome c reductase (SCR) from control and Schistosoma-infected mice was subjected for investigation. In this article, we report that SCR from Schistosoma-infected mouse showed a significant decrease in its V max and K m compared to control using both cytochrome c and 2,6-dichlorophenolindophenol as substrates. Furthermore, the kinetic studies of the purified SCR in the absence and presence of the schistosomicidal drugs praziquantel and Commiphora extract reveal that both drugs have an inhibitory action on the enzyme from the control and Schistosoma-infected mice and praziquantel changes the type of inhibition of SCR towards cytochrome c from mixed type in control to a competitive one in the case of the infection.