Resumen de Improvement of lab-scale production of microalgal carbohydrates for biofuel production

Silvia Juliana Jerez Mogollón, Laura Viviana Rueda Quiñonez, Laura Yulexi Alfonso Velazco, Andrés Fernando Barajas Solano, Crisóstomo Barajas Ferreira, Viatcheslav Kafarov

• español

  En este trabajo se estudio el mejoramiento en la concentracion de biomasa y carbohidratos (glucosa y xilosa) en Chlorella vulgaris a escala laboratorio utilizando la relacion carbono/nitrogeno; para lograr esto, se realizaron cultivos mixotroficos utilizando modificaciones en la concentracion inicial de CH3COONa (5, 10 y 20 mM), y NaNO3 (0.97, 1.94 y 2.94 mM). Los tratamientos fueron realizados a 23 1ºC, con ciclos luz.oscuridad 12h : 12h, durante 5 dias.

  Se encontro que ademas de la relacion carbono/nitrogeno, el tiempo influye en la concentracion de biomasa y carbohidratos. Al quinto dia el tratamiento 10 mM acetato: 1.94 mM nitrato alcanzo una produccion de 0.79 g/L de biomasa, 76.9 Êg/mL de xilosa y 73.7 Êg/mL de glucosa, sin embargo, al tercer dia el tratamiento 20 mM acetato: 0.97 mM nitrato produjo 1.04 g/L de biomasa, 78.9 Êg/mL de xilosa y 77.2 Êg/ mL de glucosa, mientras que el tratamiento 0 mM acetato: 2.94 mM nitrato produjo 0.55 g/L de biomasa, 40.2 Êg/mL de xilosa y 31.3 Êg/mL de glucosa.

  Al implementar la relacion carbono/nitrogeno, es posible mejorar las productividades de biomasa (de 0.55 hasta 1.04 g/L), xilosa (de 40.2 hasta 78.9 Êg/mL) y glucosa (de 31.3 hasta 77.2 Êg/mL), representando un mejoramiento de hasta 2 veces en biomasa y carbohidratos en 3 dias de cultivo.

• English

  This work studied the improvement of biomass and carbohydrate (glucose and xylose) lab.scale productivity in Chlorella vulgaris UTEX 1803 through the use of the carbon/nitrogen ratio. In order to do so, mixotrophic cultures were made by the modification of initial concentration of CH3COONa (5, 10 and 20 mM) and NaNO3 (0.97, 1.94 and 2.94 mM). All treatments were maintained at 23 1ºC, with light/dark cycles of 12h : 12h for 5 days.

  It was found that in addition to the carbon/nitrogen ratio, time also influences the concentration of biomass and carbohydrates. The treatment containing 10 mM acetate: 1.94 mM nitrate, reached a concentration of 0.79 g/L of biomass, 76.9 Êg/mL of xylose and 73.7 Êg/mL of glucose in the fifth day. However, the treatment containing 20 mM acetate: 0.97 mM nitrate produced 1.04 g/L of biomass, 78.9 Êg/mL of xylose and 77.2 Êg/mL of glucose in the third day, while in the same day the treatment containing 0 mM acetate: 2.94 mM nitrate, produced 0.55 g/L of biomass, 40.2 Êg/mL of xylose and 31.3 Êg/mL of glucose.

  The use of carbon/nitrogen ratios improved biomass productivity (from 0.55 to 1.04 g/L) as well as xylose (from 40.2 to 78.9 Êg/mL) and glucose (from 31.3 to 77.2 Êg/mL) concentration, representing an improvement of up to two times the production of both biomass and carbohydrates in only 3 days of culture.

• português

  Neste trabalho estudou.se o melhoramento na concentracao de biomassa e carboidratos (glucose e xilose) em Chlorella vulgaris a escala laboratorio utilizando a relacao carbono/ nitrogenio; para conseguir isto, realizaram.se culturas mixotroficas utilizando modificacoes na concentracao inicial de CH3COONa (5, 10 e 20 mM), e NaNO3 (0.97, 1.94 e 2.94 mM).

  Os tratamentos foram realizados a 23 1ºC, com ciclos luz.escuridao 12h : 12h durante 5 dias.

  Encontrou.se que alem da relacao carbono/nitrogenio, o tempo influi na concentracao de biomassa e carboidratos; no quinto dia o tratamento 10 mM acetato: 1.94 mM nitrato alcancou uma producao de 0.79 g/L de biomassa, 76.9 Êg/mL de xilose e 73.7 Êg/mL de glucose. Entretanto, no terceiro dia o tratamento 20mM acetato: 0.97 mM nitrato produziu 1.04 g/L de biomassa, 78.9 Êg/mL de xilose e 77.2 Êg/mL de glucose, enquanto que o tratamento 0 mM acetato:

  2.94 mM nitrato produziu 0.55 g/L de biomassa, 40.2 Êg/mL de xilose e 31.3 Êg/mL de glucose.

  Ao implantar a relacao carbono/nitrogenio, e possivel melhorar as produtividades de biomassa (de 0.55 hasta 1.04 g/L), xilose (de 40.2 ate 78.9 Êg/mL) e glucose (de 31.3 ate 77.2 Êg/mL), representando um melhoramento de ate 2 vezes em biomassa e carboidratos em 3 dias de cultura.