Rapid Screening of ASXL1, IDH1, IDH2, and c-CBL Mutations in de Novo Acute Myeloid Leukemia by High-Resolution Melting
- Autores: Mariam Ibáñez, Esperanza Such, José Cervera, Irene Luna, Inés Gómez Seguí, María López, Sandra Dolz, Eva Barragán, Óscar Fuster, Marta Llop, Rebeca Rodríguez Veiga, Amparo Avaria, Juan Silvestre Oltra Soler, M. Leonor Senent, Federico Moscardó García, Pau Montesinos, David Martínez Cuadrón, Guillermo Martín, Miguel A. Sanz
- Localización: The Journal of molecular diagnostics, ISSN 1525-1578, Vol. 14, Nº. 6, 2012, págs. 594-601
- Idioma: inglés
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Resumen
Recently, many novel molecular abnormalities were found to be distinctly associated with acute myeloid leukemia (AML). However, their clinical relevance and prognostic implications are not well established. We developed a new combination of high-resolution melting assays on a LightCycler 480 and direct sequencing to detect somatic mutations of ASXL1 (exon 12), IDH1 (exon 4), IDH2 (exon 4), and c-CBL (exons 8 and 9) genes to know their incidence and prognostic effect in a cohort of 175 patients with de novo AML: 16 patients (9%) carried ASXL1 mutations, 16 patients had IDH variations (3% with IDH1R132 and 6% with IDH2R140), and none had c-CBL mutations. Patients with ASXL1 mutations did not harbor IDH1, FLT3, or CEBPA mutations, and a combination of ASXL1 and IDH2 mutations was found only in one patient. In addition, we did not find IDH1 and FLT3 or CEBPA mutations concurrently or IDH2 with CEBPA. IDH1 and IDH2 mutations were mutually exclusive. Alternatively, NPM1 mutations were concurrently found with ASXL1, IDH1, or IDH2 with a variable incidence. Mutations were not significantly correlated with any of the clinical and biological features studied. High-resolution melting is a reliable, rapid, and efficient screening technique for mutation detection in AML. The incidence for the studied genes was in the range of those previously reported. We were unable to find an effect on the outcome.
