Resumen de Humoral immune response in lambs and goat kids inoculated with a dual vaccine against contagious agalactia

David Christian de la Fe Rodríguez, Raúl Rodríguez, Patricia Clemente Janeiro Assunçao, Ana Ramírez, María Pastora Flores Mengual, José B. Poveda Guerrero

• español

  Una vacuna polivalente frente a Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (large colony) fue elaborada utilizando cepas inactivadas seleccionadas en base a previos estudios de caracterización de las mismas. El fenol se utilizó como inactivante mientras que como adyuvante se utilizó el hidróxido de aluminio. El trabajo evaluó la respuesta immune humoral inducida en un grupo de 15 corderos Pelibuey y 8 cabritos de la raza canaria majorera, los cuales recibieron 2 dosis de vacuna a los 45 y 70 días de edad, siendo monitorizados hasta los 4 meses de vida. Los títulos de anticuerpos observados por ELISA indirecto muestran títulos menores en el grupo de corderos que en el grupo de cabritos frente a ambos antígenos, siendo estas diferencias estadísticamente significativas sólo frente a M. agalactiae.

• English

  A combined vaccine against Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (large colony type) was developed using inactivated strains selected in previous characterization studies. Phenol was used as inactivating agent and aluminium hydroxide was added as adjuvant. The present study was designed to evaluate the specific humoral immune response to these two mycoplasma species shown by lambs and goat kids. One group of 15 Pelibuey lambs and one group of 8 Canary goat kids received two injections of vaccine at the ages 45 and 70 days and were monitored until they were 120 days old. Antibody titres were determined by indirect ELISA. Lambs showed lower antibody levels (expressed as ODs) to both antigens than goat kids althought significantly differences were only registered to M. agalactiae antigen (P<0.005).