Effect of addition of concentrated proteins and seminal plasma low molecular weight proteins in freezing and thawing of equine semen
- Autores: B. Fagundes, M.F van Tilburg, G.V. Souza, J.R.C. Caiado, M.A.P Barreto, J.F.S. Silva
- Localización: Acta Biomedica Brasiliensia, ISSN-e 2236-0867, Vol. 2, Nº. 1 (Julho), 2011
- Idioma: inglés
- Enlaces
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Resumen
Difficulties in obtaining equine frozen semen with potential fertility are recognized.
This study was designed to investigate the effect of seminal plasma on frozen/thawing of eight stallion semen from different breed using the following treatments: Seminal plasma with ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender; Part of seminal plasma with proteins under 10 kDa on frozen extender; Conventional freezing, using whole seminal plasma on frozen extender. Using the parameter of 30% of seminal motility post-thawing as index of good freezability, it was verified an increased percentage of stallions that presented good freezability when semen was frozen with seminal plasma containing ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender. These results, suggested the use of seminal plasma concentrated proteins from own stallion to freezing/thawing semen.
