Resumen de Evolución radiográfica del implante de células mesenquimales de tejido adiposo diferenciadas a osteoblastos en la reparación de defectos óseos: estudio experimental en ovejas

Andrés Saldaña Díaz, Luis R. Ramos Pascua, M. López Laguna, Francisco Javier Iglesias Muñoz, José Manuel Gonzalo Orden, José Antonio Rodríguez-Altónaga Martínez, Rafael González Celador

• español

  Objetivo: Examinar la evolución radiográfica del implante de células mesenquimales derivadas de la grasa y diferenciadas a osteoblastos en defectos cavitarios del fémur ovino.

  Material y metodología: Se realizó un defecto cavitario en el cóndilo medial femoral bilateral en 22 ovejas de raza Asaaf, de 3-5 años. Se implantaron células troncales mesenquimales obtenidas del tejido graso diferenciadas a osteoblastos, plasma rico en plaquetas y heteroinjerto de hueso liofilizado. Se realizaron controles radiográficos a las 4 y 12 semanas, evaluando el crecimiento óseo según la escala de Lane y Shandu.

  Resultados: En ninguno de los grupos hubo formación o crecimiento óseo mayor del 25%. Se observó un mayor crecimiento óseo a las 12 semanas que a las 4 semanas no encontrando diferencias estadísticamente significativas entre los grupos.

  Conclusiones: El control radiográfico de la evolución de defectos óseos cavitarios tratados con células troncales diferenciadas a osteoblastos no es un método fiable ni a las 4 ni a las 12 semanas del implante

• English

  Objective: To investigate the radiographic progress of the implant of adipose tissue-derived mesenchymal cells differentiated to osteoblasts in cavitary defects of sheep femur.

  Material and Methods: A study was conducted on 100 agricultural workers performing 19 tasks (planting-6, pick-5, and other cleaning-5-3). The physiological assessment consisted of determining the physical capacity through the application of the test step. During the conduct of work, we evaluated the heart rate, oral temperature, the rate of sweats time and energy expenditure. Biomechanical analysis was performed to evaluate the position, strength and repetition of actions using the method MODSI.

  Patients and methods: A cavitary defect was performed in the bilateral medial femoral condyle in 22 Asaaf sheep aged 3-5 years. Mesenchymal stem cells obtained from adipose tissue, differentiated into osteoblasts, platelet-rich plasma and lyophilised bone heterograft, were implanted. Radiographic controls were performed at 4 and 12 weeks, evaluating bone growth according to the Lane and Shandu scale.

  Results: Bone formation or growth were not greater than 25% in any of the groups. A greater bone growth was seen at 12 weeks than at 4 weeks, with no statistically significant differences between the groups.

  Conclusions: Radiographic control of the progression of cavitary bone defects treated with stem cells differentiated to osteoblasts is not a reliable method at 4 or at 12 weeks of implantation