DNA extraction from formalin-fixed tissue: new light from the deep sea

• Autores: Ferran Palero, Sally Hall, Paul F. Clark, David Johnston, Jackie Mackenzie-Dodds, Sven Thatje
• Localización: Scientia Marina, ISSN 0214-8358, Vol. 74, Nº. 3, 2010, págs. 465-470
• Idioma: inglés
• Títulos paralelos:
  • Extracción de ADN a partir de tejido fijado en formol: nueva luz desde el mar abisal
• Enlaces
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• Resumen

  • DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR success when dealing with formalin-fixed material. Instead, amplification success from formalin-fixed tissue appears to depend on the presence of unmodified DNA in the extracted sample. A staining method that facilitates the targeting of samples with a high content of unmodified DNA is provided.