Comportamiento electroquímico de la dopamina en un electrodo de carbón vítreo modificado con laponita/glutaraldehído

• Autores: Sabino Menolasina, Begoña Martín Fernández, J. García Iñigo, Beatriz López Ruiz
• Localización: Anales de la Real Academia Nacional de Farmacia, ISSN-e 1697-4298, ISSN 0034-0618, Nº. 4 3, 2009, págs. 373-388
• Idioma: español
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• Resumen

  • In this work a new method of dopamine (DA) detection, based on glassy carbon electrode modified by adsorption on the surface with a film of laponita (cathionic clay) and glutaraldehyde (GA) is proposed. The electrochemical behaviour of the DA in a phosphate buffer solution 0,1 M, pH 6,0 of tyrosinase was studied by cyclic voltammetry technique (CV). The tyrosinase present in the solution allowed the indirect detection of DA monitoring the dopaminochrome coming from the quinone produced in the enzymatic reaction between the DA and tyrosine to �0.25 V vs. Ag/AgCl (3 M). The reduction current of the dopaminochrome was found to be proportional to the DA concentration.