Resumen de Diffusion in the antigen - antibody reaction
M. Perea Falomir, Francisco Pérez-Pla, José Luis Moreno Frigols
In previous works some indications about the importance of molecular diffusion in the antigen-antibody reaction have been reported. Polynomic plots were used for the analysis of the results; They have the advantadge of being simple, but the drawback of not giving very much information concerning the characteristics of the process. In this work the results obtained in several series of kinetics of antigen-antibody experiences were used. In most of them the antigen was labelled with I125and in some cases it was the antibody, with the same radionuclide. In all cases the reaction was followed by measuring the radioactivity of the Ag-Ab immunocomplex produced in different times. This signal is directly proportional to the concentration, so the original data are presented as Activity (represented by A) in CPM vs time (t) in minutes. The results obtained for sixteen reactions were analyzed using exclusively the integral method by means of both Stenberg and Karlsson models. Each one gives an integrated velocity ecuation that was adapted in order to be applied to the data. A Diffusion control was found in a high percentage of the studied cases.
