Intestinal uptake of amyloid ß protein through columnar epithelial cells in suckling mice

• Autores: Yasuhisa Ano, Hiroyuki Nakayama, Akikazu Sakudo, Yoriko Sawano, Masaru Tanokura, Shigeyoshi Itohara, Takashi Onodera
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 24, Nº. 3, 2009, págs. 283-292
• Idioma: español
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• Resumen

  • The mechanism of transmission of amyloid protein, especially the dynamics in the intestine, is still largely unknown. In the present study, a fusion protein (Aß-EGFP) that combined enhanced green fluorescent protein with amyloid-ß protein (Aß) was orally administered to mice before and after weaning, and the uptake and kinetics of amyloid protein within the intestine were elucidated through histopathology. Aß-EGFP was incorporated into the cytoplasm of columnar epithelial cells, rather than M cells, at 3 h after administration and thereafter. Aß-EGFP then accumulated in the crypt, Peyer's patch, and even the spleen. However, this uptake was not observed in weaned mice. These results suggest that a specific tolerant mechanism for incorporation of Aß escaped from the digestion exists during suckling periods. This age-dependent uptake is important for estimating the risk of transmission.