Experimental change on dopaminergic neurons in striatum of Parkinson disease rats

• Autores: Xiaoyu Chen, J. Li, W.Q. Qi, S.H. Shen
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 22, Nº. 10, 2007, págs. 1085-1090
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Summary. Background: Parkinson¿s disease (PD) is a common neurodegenerative disorder characterized by selective and progressive demise of dopamine-containing neurons in the midbrain. In this study, we observe the expression of c-Jun in the striatum of rats with 6-hydroxydopamine (6-OHDA)-lesions after apomorphine (APO) intraperitoneal injection (ip) in substantia nigra compacta (SNc), and to study the mechanism of the rotations behavior. Design: The 6-OHDA was unilaterally injected into rat right SNC. The APO- induced abnormal rotations were investigated on the 1st, 4th, 7th, 14th, 21st days after lesion, respectively. Meanwhile dopaminergic degeneration and c-Jun expression were observed with microscope. Nissl¿s body staining and immunohistochemical method (ABC) were employed to study the changes of tyrosine hydroxylase (TH) and c-Jun in DA neurons. Results: We found that the number of dopaminergic neurons decreased gradually in the lesioned site and those neurons¿ electron-microscopic structure was severe damaged. There were over 75% of dopaminergic neurons lost, contralateral rotations over 7 turns per minute and c-Jun expressing in the ipsilateral striatum. Conclusion: Dopaminergic neurons deletion may be linked to upregulation of c-Jun. Histol Histopathol 22, 1085-1090 (2007)