Resumen de The effects of different anaesthetic treatments on the adreno-cortical functions and glucose levels in NZW rabbits.
Juan Carlos Illera del Portal, Alfredo González Gil, Gema Silván Granado, Mariano Illera Martín
- español
Se estudian los efectos de 5 anestésicos sobre las concentraciones de corticosterona, cortisol y glucosa en el conejo NZW. Se utilizan 60 animales, divididos en 6 grupos (n= 10 por grupo): control (inyección de solución salina iv), fentanil/droperidol (1 mg/kg sc), ketamina (10 mg/kg iv) junto a xilazina (3 mg/kg iv) o diacepán (2 mg/kg iv), pentobarbital (30 mg/kg iv) y tiopental (20 mg/kg iv). Las concentraciones plasmáticas de glucocorticoides se miden mediante la técnica EIA de competición y la glucosa con un autoanalizador. Las muestras de sangre se recogens a los 0, 10, 30, 60, 120 min y 24 horas tras la administración de los anestésicos/solución salina. En los grupos tratados con pentobarbital o fentanil/droperidol se observa una disminución significativa de los glucocorticoides plasmáticos a los 10-60 min, mientras que la administración de ketamina/diacepán o del tiopental estimula la liberación de glucocorticoides, principalmente durante la recuperación. Sin embargo, tras la administración de ketamina/xilazina no se observan cambios en los niveles de glucocorticoides, excepto un aumento significativo de cortisol a los 60-120 min. Los niveles de glucosa aumentan significativamente tras la administración de ketamina/diacepán y principalmente, de ketamina/xilazina. Los datos presentes sugieren que la ketamina/xilazina apenas afecta a los niveles de glucocorticoides y alcanza un adecuado nivel de anestesia quirúrgica, por lo que sería el anestésico de elección, aunque el efecto hiperglicémico tras el tratamiento ha de ser considerado en cualquier experimento realizado en conejos
- English
The effects of five anaesthetics on the corticosterone, cortisol and glucose concentrations were investigated in the NZW rabbit. Sixty animals were assigned to 6 treatment groups (n= 10 per group): control ( iv saline solution injection), ketamine (10 mg/kg iv) with either xylazine (3 mg/kg iv) or diazepam (2 mg/kg iv), pentobarbitone (30 mg/kg iv), thiopentone (20 mg/kg iv) and fentanyl/droperidol (1 mg/kg sc). Plasma glucocorticoids were measured by competitive enzymeimmunoassay EIA and glucose by an autoanalyzer, previously validated for this species in both cases. Blood samples were obtained at 6 time-points: before injection, at 10, 30, 60, 120 min and 24 h after injection of the anaesthetics/saline. A significant decrease of plasma glucocorticoids at 10-60 min was observed in the pentobarbitone and fentanyl/droperidol groups, whereas the administration of ketamine/diazepam or thiopentone stimulated plasma glucocorticoid release, principally in the recovery period. However, in the ketamine/xylazine group no changes were observed in the glucocorticoid levels, except for a significative increase of cortisol at 60-120 min. Glucose levels significantly increased after ketamine/diazepam administration and principally, after ketamine/xylazine treatment. The present data suggest that ketamine/xylazine has little effect on glucocorticoid levels and provides an adequate level of surgical anaesthesia, hence it would be the anaesthetic of choice, although the hyperglycaemic effect after injection has to be considered for any experimental procedures in rabbits.
