The MAP kinase pathways mediate leptin-induced lipolysis.

• Autores: A. Marti, M. Aguado, E. Martínez Anso, José Alfredo Martínez Hernández
• Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 57, Nº. 2, 2001, pág. 107
• Idioma: inglés
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• Resumen

  • It has been reported that leptin has a direct autocrine/paracrine effect on the lipolytic rate of white adipocytes and that the mechanism for leptin signal transduction following the binding to its receptor may involve different signalling pathways. The present study investigated whether the mechanism of leptin-induced lipolysis involves the MAP kinase pathways. The lipolytic rate was assessed through glycerol release after incubation of the adipocytes with leptin following a pretreatment with the MAP kinases inhibitors sodium salicylate, NaSal, or PD098059. After preincubation with 50 or 100 mM NaSal, a significant reduction in the leptin-induced lipolysis was found (-27%, p< 0.01). Moreover, in the presence of PD098059 at either 50 or 100 mM concentration, the lipolytic activity of the leptin was dramatically reduced (-54%, p<0.01) compared to the effect of the leptin alone. We conclude that signalling through members of the MAP kinase family is an intermediate step in leptin-induced lipolysis