Verapamil sensitisation to alkaloids on colchicine-selected human colon adenocarcinoma cells.

• Autores: Miguel José Ruiz Gómez, Antonio Souvirón Rodríguez, Luisa Gil Carmona, Manuel Martínez Morillo
• Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 57, Nº. 4, 2001, págs. 343-344
• Idioma: inglés
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• Resumen

  • The main strategy to overcome the multidrug resistance associated with P-glycoprotein (P-gly) focuses on inhibiting the drig efflux caused by this protein. Verapamil reverses drug resistance by competitively inhibiting the binding of P-gly and cytotoxic drugs. We investigate verapamil sensitisation to drugs with the same mechanism of action on a multidrug resistant colon carcinoma cell line selected with colchicine. The effect of verapamil was performed by the exposure of colon cells to colchicine, vinblastine and vincristine in the presence of 10 mg/ml verapamil for 1 hour. The highest sensitisation value obtained was 90.51 % for verapamil increases sensitivity to 0.5 mg/ml colchicine, the drug presence during the selection. Verapamil increases sensitivity to 8.0 mg/ml vinblastine by 60 % and by 63.58 % for 25.0 mg/ml vincristine. The data obtained suggest that verapamil may sensitise these colon adenocarcinoma cells to drugs with the same mechanisms of action as that of the drug presence during the selection, although with different efficacy