Resumen de Cytokine effect on intestinal galactose absorption.
C. Bértolo, J. Barrenetxe, Ana María Barber Cárcamo, María Pilar Lostao Crespo
In this work, the effect of the cytokines TNF-a and IL-1b on the intestinal galactose absorption is investigated. The absorption studies were performed using everted rings of rat intestine which were incubated in galactose (C14)-containing saline solution for 5 or 30 min. Different concentrations of TNF-a (1-100 ng/ml) or IL-1b (0.5-25 ng/ml) were added to the incubation saline solution. At 10, 50 and 100 ng/ml concentrations, TNF-a inhibits galactose absorption by 30% and at 25 ng/ml, there is a reversal of the inhibition. This biphasic effect of TNF-a on galactose absorption is observed at both 5 and 30 min incubation. IL-1b inhibited galactose uptake at all tested concentrations after 30 min incubation, although the effect was not concentration dependent. Both TNF-a and IL-1b inhibits only the phloridzin-sensitive component of the intestinal galactose absorption, indicating that they alter SGLT1 functionality.
