Resumen de Conservation via "in vitro" propagation of endangered species from Grazalema Natural Park (early results)
M. Cantos, Rafael Zárate, Antonio Troncoso de Arce
In vitro propagation under controlled culture conditions of the endangered species from Grazalema Natural Park Atrop aetic ilIk, Centranthus nevadensis Boiss, Echinosparttern aigibicum Talavera & Aparicio, Lavaiera maritima Gouan and Phiomis nzargaritae Aparicio Silvestre is being investigated. Initially, gemination trials were performed with the species A. baetica, Ea aigibicum and L. maritima but not with C. nevadensis (due to lack of seeds) and P. margaritae (a hybrid which does not set seeds). Germination was satisfactory for all the species in the two culture media used (MS and Troncoso in both cases with 3% sucrose) with a higher response and better seedling growth of A. baetica and E. algibicion. The establishment of micropropagation protocols for all these species has been initiated employing as source of plant material apical and axillary buds, rhizome (only with .P margaritae) petioles and leaves. At this early stage of the investigation, Ai baetica has provided promising and encouraging results in all the micropropagation stages being MS basal salts with 3% sucrose supplemented with different combinations of BAP and NAA a positive nutrient medium. Moreover, adaptation of regenerated A. bactica plantlets to greenhouse conditions has been achieved. The response obtained with the other species has been less promising due to high contamination rates and to response to the culture media used. However, this requires further studies which are currently being undertaken (project no. AMB94-1372 funded by CICYT).
