Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes

• Autores: Ingo H. Gorr, Alexandra Reis, Dominik Boos, Martin Wühr, Suzanne Madgwick, Keith T. Jones, Olaf Stemmann
• Localización: Nature: Cell biology, ISSN 1465-7392, Nº. 9, 2006, págs. 1035-1037
• Idioma: inglés
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• Resumen

  • Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase–Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.