Origin of the main locally adapted sheep breeds of Brazil: a RFLP-PCR molecular analysis

• Autores: S.R. Paiva, D.A. de F. Paiva, S. T. R. Castro, M. S. M. Albuquerque, A. A. Egito, C. McManus, A. S. Mariante, J.A. Dergam, V.C. Silverio
• Localización: Archivos de zootecnia, ISSN-e 1885-4494, ISSN 0004-0592, Vol. 54, Nº 206-207, 2005, págs. 395-399
• Idioma: español
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• Resumen

  • Likewise other domestic animals, sheep were introduced in Brazil by the Portuguese settlers. Characterizing the geographic origins and the extent of variability of the genetic resources must also precede any conservation and management program. Recent work on variation of mtDNA of Ovis aries indicates that this species may be subdivided in two haplogroups, a European and an Asian. These haplogroups may be readily distinguished by RFLP-PCR (Restriction Fragment Length Polymorphism- Polymerase Chain Reaction) analysis. This approach was employed to recognize the origins of the main Brazilian breeds. A 1052 Kb fragment of the mitochondrial cytochrome oxidase I gene region was amplified in 248 specimens of the Santa Inês, Crioula Lanada, Rabo Largo, Morada Nova, Somali and Bergamasca breeds. The patterns were assigned with HinfI recognition site to nucleotide positions 5562-5566 in the COI gene. All animals were of European descent. However, these results also applied to the Brazilian breeds Somali and Morada Nova, considered as from African origin. The African breeds may share the same evolutionary history as the European breeds. A further analysis of mtDNA sequences and microsatellites will include more Brazilian, European and African breeds