Corrientes colinérgicas en oocitos de "Xenopus" trasplantados con membranas de cerebro humano

• Autores: Esteve Ros, Laura Texidó, Jordi Marsal, Jordi Aleu, Piedad Navarro
• Localización: Anales de la Real Academia Nacional de Farmacia, ISSN-e 1697-4298, ISSN 0034-0618, Nº. 4 4, 2005, págs. 821-834
• Idioma: español
• Títulos paralelos:
  • Cholinergic currents in Xenopus oocytes transplanted with human brain membranes
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• Resumen

  • Cholinergic human brain currents were recorded in Xenopus laevis oocytes transplanted with human cerebral membranes from two different zones, the frontal cortex and the hippocampus. The recorded currents were supported by the nicotinic or the muscarinic acetylcholine receptor. We tested the effects of a number of several nicotinic agonists acetylcholine, nicotine and 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP), and the nicotinic receptor antagonists a-bungarotoxin and d-tubocurarine on the transplanted oocytes. We detected four kinds of nicotinic current kinetics. The differences in the amplitude and in the total electric charge of the currents elicited by various agonists at a range of holding potentials were not significant, except in the case of DMPP at a holding potential of -90 mV. Our results indicate that á4â2, á3â4 and á7 are the main nicotinic receptors in human brain.