Resumen de Histochemical analysis of glycoconjugates in the domestic cat testis
S. Desantis, G. Ventriglia, D. Zubani, M. Deflorio, P. Megalofonou, F. Acone, A. Zarrilli, G. Palmieri, Gregorio de Metrio
Summary. The localization and characterization of oligosaccharide sequences in the cat testis was investigated using 12 lectins in combination with the ß-elimination reaction, N-Glycosidase F and sialidase digestion. Leydig cells expressed O-linked glycans with terminal alphaGalNAc (HPA reactivity) and N-glycans with terminal/internal aMan (Con A affinity). The basement membrane showed terminal Neu5Acalpha2,6Gal/GalNAc, Galß1,3GalNAc, alpha/ßGalNAc, and GlcNAc (SNA, PNA, HPA, SBA, GSA II reactivity) in O-linked oligo-saccharides, terminal Galß1,4GlcNAc (RCA120 staining) and alphaMan in N-linked oligosaccharides; in addition, terminal Neu5acalpha2,3Galß1,4GlcNac, Forssman pentasaccharide, alphaGal, alphaL-Fuc and internal GlcNAc (MAL II, DBA, GSA I-B4, UEA I, KOH-sialidase-WGA affinity) formed both O- and N-linked oligosaccharides. The Sertoli cells cytoplasm contained terminal Neu5Ac-Galß1,4GlcNAc, Neu5Ac-ßGalNAc as well as internal GlcNAc in O-linked glycans, alphaMan in N-linked glycoproteins and terminal Neu5Acalpha2,6Gal/GalNAc in both O- and N-linked oligosaccharides. Spermatogonia exhibited cytoplasmic N-linked glycoproteins with aMan residues. The spermatocytes cytoplasm expressed terminal Neu5acalpha2,3Galß1,4GlcNAc and Galß1,3GalNAc in O-linked oligo-saccharides, terminal Galß1,4GlcNAc and alpha/ßGalNAc in N-linked glycoconjugates. The Golgi region showed terminal Neu5acalpha2,3Galß1,4GlcNac, Galß1,4GlcNAc, Forssman pentasaccharide, and alphaGalNAc in O-linked oligosaccharides, alphaMan and terminal ßGal in N-linked oligosaccharides. The acrosomes of Golgi-phase spermatids expressed terminal Galß1,3GalNAc, Galß1,4GlcNAc, Forssmann pentasaccharide, alpha/ßGalNAc, alphaGal and internal GlcNAc in O-linked oligosaccharides, terminal alpha/ßGalNAc, alphaGal and terminal/internal alphaMan in N-linked glycoproteins. The acrosomes of cap-phase spermatids lacked internal Forssman pentasaccharide and alphaGal, while having increased alpha/ßGalNAc. The acrosomes of elongated spermatids did not show terminal Galß1,3GalNAc, displayed terminal Galß1,4GlcNAc and alpha/ßGalNAc in N-glycans and Neu5Ac-Galß1,3GalNAc in O-linked oligosaccharides. Histol Histopathol 21, 11-22 (2006)
