Resumen de Three brainstem nuclei of the rat share cytoplasmic Fos-like immunoreactivity.
T. Hernández Gil, Francisco Martínez Soriano, Amparo Ruiz Torner, Alfonso Amador Valverde Navarro, Francisco Eliseo Olucha Bordonau
We investigated the neuronal origin of Fos-like immunopositive nervous fibers re c e n t l y observed in our laboratory. Two different anti- Fos antisera, from Genosys and from Calbiochem, were used. They were tested in both non-stimulated and noxious-stimulated rats. A strong and Golgi-like immunoreactivity in the cytoplasmic compartment of neurons was detected only when using anti-Fos from Genosys. Cytoplasmic labelled neurons were localized at the level of nucleus O, central gray pars alpha, and nucleus raphe pontis. This non-nuclear labelling remained constant irrespective of whether the noxious stimulation was applied or not. These results indicate that the neurons of these three brainstem nuclei share the property of being labelled by the Genosys polyclonal anti- Fos antibody, suggesting a closer relationship among them than reported to date.
