Effect of recombinant human GH and GHRH on plasma metabolite levels in rainbow trout (Oncorhynchus mykiss)

• Autores: Salvador Zamora Navarro, M. J. Dato Gómez, Pilar Mendiola López, Jorge de Costa Ruiz, María Dolores Hernández Llorente
• Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 60, Nº. 3, 2004, págs. 211-218
• Idioma: inglés
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• Resumen

  • The aim of the present work was to study metabolic changes in rainbow trout treated with preparations of human recombinants of growth hormone (rhGH) and GH-releasing hormone (rhGHRH), by analysing time course variations in plasma metabolite levels. Trouts were given the hormones by intraperitoneal injection in a single dose (rhGH: 0.5µg/g b.w.; rhGHRH: 0.06µg/g b.w.) or as a weekly injection over a period of 4 weeks. The concentrations of glucose, lactate, free fatty acids and glycerol were determined in plasma immediately before injection and 1, 3, 6, 12, 24, 48 and 168 hours post-injection. Results indicate that glucose and lactate levels increase, while slight variations in free fatty acids was observed after the injection of rhGH and rhGHRH.