Abstract. The study focused on the life cycle of Chondracanthus chamissoi, characterized by its isomorphic triphasic phase and spore propagation. The effect of phytohormones in alternative culture media on the settlement and growth of gametophytes was evaluated. Tetraspores were inoculated on polypropylene ropes and exposed to six treatments with different concentrations of a hormonal solution (gibberellins, auxins, cytokinins). Over 10 weeks, the number of settled spores, disc density, and microthallus size were measured. The results showed increased growth with higher concentrations of phytohormones, the treatment with 100% phytohormones (T1) produced the highest amount of developed discs (133 ± 9 mm-2), which confirms its positive effect on the morphogenesis of C. chamissoi, while the treatment without phytohormones (T6) showed the lowest amount of developed discs (25 ± 10 mm-2) suggesting their usefulness in the propagation of this economically important species.
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