Efeitos letais e subletais do bioinseticida à base de Autographa californica multiple nucleopolyhedrovirus (AcMNPV) em Rachiplusia nu (Lepidoptera: : Noctuidae) com resistência à soja Cry1Ac

• Venicius Ernesto Pretto ^[1] ; Daniela Neves Godoy ^[1] ; Amanda de Freitas Wendt ^[1] ; Luiz Francisco Warpechowski ^[1] ; Eduardo Argenta Steinhaus ^[1] ; Oderlei Bernardi ^[1]
  1. [1] Universidade Federal de Santa Maria

     Universidade Federal de Santa Maria

     Brasil

     
• Localización: Ciencia rural, ISSN 0103-8478, Vol. 55, Nº. 12, 2025
• Idioma: portugués
• Títulos paralelos:
  • Lethal and sublethal effects of an Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-based biopesticide in Rachiplusia nu (Lepidoptera: : Noctuidae) with resistance to Cry1Ac soybean
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• Resumen
  • English

    Autographa californica multiple nucleopolyhedrovirus (AcMNPV: Baculoviridae: Alphabaculovirus) é o ingrediente ativo de um biopesticida comercial para o controle de Rachiplusia nu (Guenée, 1852) (Lepidoptera: Noctuidae) em soja no Brasil. Neste estudo, investigou-se os efeitos letais e subletais do AcMNPV infectando diferentes estágios larvais de R. nu com resistência à toxina Cry1Ac expressa na soja. Inicialmente, selecionou-se uma população de R. nu com resistência a Cry1Ac e, posteriormente, larvas de primeiro (L1) e segundo (L2) instares foram expostas a folhas de soja expressando Cry1Ac tratadas com a dose de campo de AcMNPV (1,13 × 109 corpos de oclusão (OBs)/L) ou 50% da dose de campo (5,63 × 108 OBs/L). Os resultados revelaram que <31% das larvas L1 e L2 sobreviveram em ambas as doses de AcMNPV (dose de campo e 50% da dose de campo) aos 15 dias após a infecção e <5% sobreviveram até a fase adulta. Não foram detectados efeitos subletais de AcMNPV no tempo de desenvolvimento dos insetos sobreviventes. O biopesticida à base de AcMNPV pode ser considerado uma nova opção para controle de R. nu em soja no Brasil.

  • português

    Autographa californica multiple nucleopolyhedrovirus (AcMNPV: Baculoviridae: Alphabaculovirus) is the active ingredient of a commercial biopesticide registered for managing Rachiplusia nu (Guenée, 1852) (Lepidoptera: Noctuidae) in soybean in Brazil. In this study, we investigated the lethal and sublethal effects of AcMNPV infecting different larval stages of R. nu with resistance to the Cry1Ac toxin expressed in soybean. Initially, a Cry1Ac-resistant strain of R. nu was selected, and the first (L1) and second (L2) instar larvae from this strain were fed on Cry1Ac soybean leaves treated with the field-dose of AcMNPV (1.13 × 109 occlusion bodies (OBs)/L) and 50% of the field dose (5.63 × 108 OBs/L). The results revealed that <31% of L1 and L2 larvae survived in both doses of AcMNPV (field dose and 50% of the field dose) after 15 days post-infection and <5% survived until the adult stage. No pronounced sublethal effects of AcMNPV were identified on the developmental time of surviving insects. In summary, the AcMNPV-based biopesticide may be considered a valuable eco-friendly option for controlling R. nu in soybean in Brazil