Oxidative stress markers 8-OHdG, Trx-1, and Prx6 are expressed in seminoma and are connected to TXNDC2, 3, and 6 expression levels
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Inka Rantala [1] ; Hanna-Riikka Teppo [1] ; Jonas Händelin [1] ; Janette Kemppainen [1] ; Riina K. Ollikainen [1] ; Outi Kuittinen [2] ; Milla E.L. Kuusisto [1]
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[1] University of Oulu
University of Oulu
Oulu, Finlandia
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[2] University of Eastern Finland
University of Eastern Finland
Kuopio, Finlandia
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- Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 40, Nº. 10, 2025, págs. 1607-1615
- Idioma: inglés
- Enlaces
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Resumen
Testis-specific thioredoxins (TXNDCs) and oxidative stress have not been studied before in seminoma. We studied the immunohistochemical (IHC) expression of 8-OHdG, Prx6, Trx-1, TXNDC2, 3, and 6 in 25 testicular seminoma and 24 control samples. We retrospectively collected patient details and treatments from hospital records and combined them with IHC results.
In normal testis, IHC expression of 8-OHdG, Prx6, TXNDC2, and 3 was higher than in seminoma samples (p<0.001 each), and the absence of n8-OHdG (p=0.002) and nTXNDC2 expression (p=0.044) was clinically associated with elevated lactate dehydrogenase levels.
On the contrary, Trx-1 and TXNDC6 were overexpressed in seminoma samples (p=0.035 and p<0.001, respectively), and TXNDC6 was negatively associated with age over 40 (p=0.036). Concordant with the IHC expression, PRDX6 mRNA levels were downregulated with a fold change of -2517.4 (p<0.001), and TXN mRNA levels were upregulated with a fold change of 11637.4 (p=0.008) in seminoma samples compared with healthy controls. The oxidative stress markers studied had a correlation between the nuclear and cytoplasmic localization, a mutual correlation in expression between different markers, and mutual protein-protein interactions according to STRING Enrichment analysis.
Our results show that oxidative stress markers and their expression in seminoma differ from that in normal testis tissue. Trx-1 and cTXNDC6 are seemingly overexpressed in seminoma, which might indicate their relevance in seminoma biology.
