Characterization of Virulence Genotypes and Prevalence of Vibrio parahaemolyticus from Fish Samples Collected from Thoothukudi Fish Landing Centres, India

• Selvamathi Veerabhadran Ragul ^[2] ; Fathiraja Parthiban ^[2] ; Rajendran Shalini ^[2] ; Sivasankar Panchavarnam ^[2] ; Thandapani Muthulakshmi ^[3] ; Mohamed Mansoor ^[2] ; Stephen Jerusha ^[1] ; Selvam Krishnakumar ^[2]
  1. [1] Hebrew University of Jerusalem

     Hebrew University of Jerusalem

     Israel

     
  2. [2] Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Fisheries College and Research Institute, Thoothukudi, Tamil Nadu, India
  3. [3] ICAR-Central Institute of Fisheries Technology, Cochin, Kerala, India

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• Localización: Thalassas: An international journal of marine sciences, ISSN 0212-5919, Vol. 41, Nº. 2 (June 2025), 2025
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Vibrio parahaemolyticus is a pathogen of signifcant importance in the seafood sector, and the increased incidence of antimicrobial resistance in this pathogen poses a serious threat to seafood safety. To investigate this issue, a total of 90 samples comprising 78 fshes (46 fnfsh and 32 shellfsh) and 12 water samples were collected from two fsh landing centres in Thoothukudi, Tamil Nadu, India: the motorized boat landing centre at Therespuram (LC1) and the mechanized boat landing centre at the Fishing Harbour (LC2). A total of 126 suspected V. parahaemolyticus colonies were isolated among which biochemical testing identifed 37 isolates (29%) as presumptive V. parahaemolyticus. Among them, 29 (78.4%) were positive for toxR gene PCR confrmation, while only 8 exhibited the Kanagawa phenomenon. The shrimp samples showed a higher prevalence of V. parahaemolyticus (10%) than the crabs (6.6%), fnfshes (5.5%), and water (1.1%) samples. The prevalence of virulence factors in V. parahaemolyticus are as follows: tdh 2 (7%), trh 7 (24%), tlh 29 (100%) and T3SS1 genes 25 (86%). Antimicrobial susceptibility testing (AST) was done using 11 antibiotics belongs to 10 diferent classes.

    93% of the isolates were resistant to trimethoprim, and 14% and 7% of isolates were resistant to cefoxitin and ceftazidime respectively. Higher Multiple Antibiotic Resistant (MAR) index values (>0.2) were observed in 28% of the isolates. This study underscores the critical role of routine monitoring, strict hygiene practices, and correct seafood handling procedures in maintaining food safety.