Optimizing the preparation of paraffin sections from stallion testes

• Nairag Asgenbaatar ^[1] ; Minna Yi ^[1] ; Xisheng Wang ^[1] ; Tseweendolmaa Ulaangerel ^[1] ; Yingchao Shen ^[1] ; Xin Wen ^[1] ; Ming Du ^[1] ; Xiaoling Dong ^[2] ; Yibeeltu Mengkh ^[3] ; Manglai Dugarjav ^[1] ; Gerelchimeg Bou ^[1]
  1. [1] Inner Mongolia Agricultural University

     Inner Mongolia Agricultural University

     China

     
  2. [2] Da Bei Nong Group Rumination Technology Rumination Academy, Haidian District, Beijing, China
  3. [3] Xilingol League Animal Husbandry, Work Station, Inner Mongolia Xilinhot, China

  Mostrar afiliaciones +
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 40, Nº. 3, 2025, págs. 335-342
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • The preparation of paraffin sections is an important experimental technique in animal histological research, and key factors that determine the quality of a section include the dehydration time, waxing time, and drying temperature of the paraffin section. Paraffin sections obtained from testis tissue of adult horses exhibited higher quality with clear tissue structure and complete cell morphology after they underwent gradient dehydration for 6 hours, were immersed in wax for 60 minutes, and were dried in a 75-degree oven for 15 minutes. The detailed, optimized procedures that are developed in the current study may simplify histological experiments and research on equine testes.