BioTechniques

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  Año 2001, Vol. 30, Número 2

  • Chromosome Walking by Cloning of Distinct PCR Fragments

    B. Kneidinger, M. Graninger, P. Messner

    pág. 248

    

  • Scaling Up the Ligase Chain Reaction-Based Approach to Gene Synthesis

    F. M. Chalmers, K. M. Curnow

    págs. 249-251

    

  • Improvement of the pBRINT-T~s Plasmid Family to Obtain Marker-Free Chromosomal Insertion of Cloned DNA in E. coli

    G. Gosset, S. Le Borgne, B. Palmeros, F. Bolivar

    págs. 252-257

    

  • Polyacrylamide Gel as a Matrix for the Delivery of a Layer or Coat of Other Molecules

    M. Deshpande, D. Mitra, P. B. Parab

    págs. 258-263

    

  • Running Gels Backwards to Select DNA Molecules Larger than a Minimum Size

    M. W. Frohlich, D. S. Parker

    págs. 264-265

    

  • Method for the Immunological Detection of Silver-Stained Proteins on Nitrocellulose Membranes

    M. Scharfke, H. Baumeister, W. Meyerhof

    págs. 266-271

    

  • DNA Isolation from Chloroform/Methanol-Treated Mycobacterial Cells without Lysozyme and Proteinase K

    A. Mve-Obiang, M. Mestdagh, F. Portaels

    págs. 272-282

    

  • A Brief History of Time-Lapse

    S. Paddock

    págs. 283-289

    

  • Immunomagnetic DNA Aptamer Assay

    P. D. Rye, K. Nustad

    págs. 290-295

    

  • Two-Hybrid Selection Assay to Identify Proteins Interacting with Polymerase II Transcription Factors and Regulators

    M. Petrascheck, F. Castagna, A. Barberis

    págs. 296-303

    

  • Inside or Outside: Detecting the Cellular Location of Bacterial Pathogens

    R. A. Edwards, S. R. Maloy

    págs. 304-310

    

  • Protein Aggregation Mediated by Cysteine Oxidation During the Stacking Phase of Discontinuous Buffer SDS-PAGE

    M. K. Crow, N. Karasavvas, A. H. Sarris

    págs. 311-317

    

  • High-Throughput Methods for Detection of Genetic Variation

    A.-L. Borresen-Dale, V. N. Kristensen, D. Kelefiotis, T. Kristensen

    págs. 318-333

    

  • High-Throughput Conformation-Sensitive Gel Electrophoresis for Discovery of SNPs

    L. Baum, K. W. Choy, D. S. C. Lam, C. P. Pang, Y. F. Leung, P. O.-S. Tam, W. C. Tong

    págs. 334-341

    

  • High-Resolution Detection of Loss of Heterozygosity of Dinucleotide Microsatellite Markers

    R. N. Hourihan, G. C. O Sullivan, J. G. Morgan

    págs. 342-347

    

  • High-Throughput AFLP Analysis Using Infrared Dye-Labeled Primers and an Automated DNA Sequencer

    R. R. Sederoff, R. W. Whetten, A. A. Myburg, D. L. Remington, D. M. O Malley

    págs. 348-357

    

  • Melting Curve Analysis of SNPs (McSNP): A Gel-Free and Inexpensive Approach for SNP Genotyping

    S. Dios, K. Hiester, B. Su, C. Bonilla, L. Jin, M. D. Shriver, J. M. Akey, D. Sosnoski, E. Parra

    págs. 358-367

    

  • Fabrication of DNA Microarrays Using Unmodified Oligonucleotide Probes

    D. R. Call, D. P. Chandler, Fred J. Brockman

    págs. 368-379

    

  • The RITE Assay: Identifying Effectors that Target the Transcription Machinery Using Phage Display Technology

    J. M. Mazzarelli, R. P. Ricciardi

    págs. 380-387

    

  • Lateral View Flow System for Studies of Cell Adhesion and Deformation under Flow Conditions

    L. L. Munn, J. Yuan, R. J. Melder, R. K. Jain

    págs. 388-395

    

  • Sensitive Measurement of Polyethylene Glycol-Modified Proteins

    N. Tsai, T.-L. Cheng, S. R. Roffler

    págs. 396-403

    

  • Selection of scFv Phages on Intact Cells Under Low pH Conditions Leads to a Significant Loss of Insert-Free Phages

    A. Engert, S. Barth, M. K. Tur, M. Huhn, S. Sasse

    págs. 404-413

    

  • Amplification and Direct Sequence Analysis of the 23S rRNA Gene from Thermophilic Bacteria

    A. Ibrahim, J. Hofman-Bang, B. K. Ahring

    págs. 414-421

    

  • Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System

    J. J. S. Cadwell, L. W. K. Chung, C. Kao, T. A. Gardner, S.-C. Ko, L. Yang

    págs. 422-427

    

  • Detection and Identification of Virulence Factors in Yersinia pestis Using SELDI ProteinChip® System

    E. Garcia, V. Thulasiraman, S. L. McCutchen-Maloney, V. L. Motin

    págs. 428-432