BioTechniques

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  Año 2003, Vol. 34, Número 4

  • BioSpotlight

    págs. 673-675

    

  • Efficient cloning of SAGE tags by blunt-end ligation of polished concatemers

    A. Koehl, E. Friauf, H.G. Nothwang

    págs. 692-694

    

  • Improved host range selection for recombinant modified vaccinia virus ankara

    G. Sutter, C. Staib, M. Löwel, V. Erfle

    págs. 694-700

    

  • Genomic DNA labeling for hybridization with DNA arrays

    P. Amon, I. Ivanov

    págs. 700-704

    

  • Use of DHPLC for rapid screening of recombinant clones

    E. Conti, B. Dallapiccola, A. Colosimo, V. Guida, E. Flex

    págs. 706-708

    

  • Optical thermal cycler for use as a fluorimetric plate reader to estimate DNA concentrations

    J.M. González, C. Saiz-Jiménez

    págs. 710-712

    

  • Modified 3'-end amplification PCR for gene expression analysis in single cells

    T Heams, J.-J. Kupiec

    págs. 712-716

    

  • Increased throughput of BAC/PAC insert size determinations by stacking gels during pulsed-field gel electrophoresis

    F. Martins-Wess, T. Leeb

    págs. 718-720

    

  • Background priming during reverse transcription by oligo(dT) carried over from mRNA isolation

    C. Piché, J.P. Schernthaner

    págs. 720-724

    

  • Quantitative analysis of transcript accumulation from genes duplicated by polyploidy using cDNA-SSCP

    R.C. Cronn, K.L. Adams

    págs. 726-734

    

  • RNA interference by production of short hairpin dsRNA in ES cells, their differentiated derivatives, and in somatic cell lines

    M. Zernicka-Goetz, D.M. Glover, J.B. Grabarek, F. Wianny, B. Plusa

    págs. 734-744

    

  • Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses

    T. Tahira, K. Hayashi, Shingo Baba, Y. Kukita, K. Higasa

    págs. 746-750

    

  • 3' RACE walking along a large cDNA employing tiered suppression PCR

    J.A. Marshall Graves, D.J. Park, A.J. Pask, M.B. Renfree

    págs. 750-756

    

  • Construction of cDNA libraries for highly efficient DNA sequencing from the 3' end of expressed genes

    G.K. Fu, L.L. Stuve

    págs. 758-766

    

  • Full-length cDNA synthesis for long-distance RT-PCR of large mRNA transcripts

    P.R. Hawkins, P. Jin, G.K. Fu

    págs. 768-773

    

  • Shedding light and electrons on the Lamellipodium: imaging the motor of crawling cells

    E. Vignal, G.P. Resch

    págs. 780-789

    

  • Understanding bias in microbial community analysis techniques due to rrn operon copy number heterogeneity

    L.D Crosby, C.S. Criddle

    págs. 790-802

    

  • Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR

    K. Rudi, H.K. Nogva, S.M. Drømtorp, H. Nissen

    págs. 804-813

    

  • Facilitated loading of horizontal gels using a capillary comb loader

    A. Safarpour, A. Christie, J. Pel, S. Chow, A. Marziali, J. Thompson, T. Pugh, K. Guggenheimer

    págs. 814-818

    

  • High-throughput DNA extraction method suitable for PCR

    J.J. Burke, Z. Xin, J.P. Velten, M.J. Oliver

    págs. 820-826

    

  • Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies

    C.P. Mîinea, G.E. Lienhard

    págs. 828-831

    

  • Ion-trap mass spectrometry used in combination with gas chromatography for high-resolution metabolic flux determination

    M.I. Klapa, J.-C. Aon, G. Stephanopoulos

    págs. 832-849

    

  • Development and characterization of the NanoOrange (R) protein quantitation assay: a fluorescence-based assay of proteins in solution

    L.J. Jones, R.P. Haugland, V.L. Singer

    págs. 850-861

    

  • Parallel DNA template preparation using a vacuum filtration sample transfer device

    J. Forsell, A.-L. Schiller, A. Alderborn, L.M. Berg, J. Dunker, U. Larsson, D. Petersson

    págs. 862-868

    

  • New products for bioresearch

    págs. 870-878

    

  • Index to advertisers

    pág. 879