BioTechniques

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  Año 2003, Vol. 35, Número 3

  • Scientists: profile

    pág. 419

    

  • BioSpotlight: in this issue

    págs. 421-423

    

  • BioSpotlight: in the recent literature

    pág. 425

    

  • Proteomics: evolution of the technology

    S.D. Patterson

    págs. 440-444

    

  • Highly sensitive microplate ß-galactosidase assay for yeast two-hybrid systems

    L. Brouchon-Macari, M.-C. Joseph, M.-C. Dagher

    págs. 446-448

    

  • Quantitative recovery of immunoreactive proteins from clinical samples following RNA and DNA isolation

    K. Nicolaides, S. Banerjee, A. Smallwood, A.E. Chambers

    págs. 450-456

    

  • Selection of optimal internal controls for gene expression profiling of liver disease

    S. Kim, T. Kim

    págs. 456-460

    

  • Large-scale phagemid conversion on solid medium

    J.-L. Evrard, F. Béna

    págs. 462-464

    

  • Viability assay for Dictyostelium for use in drug studies

    H. Alexander, A.N. Vomund, S. Alexander

    págs. 464-470

    

  • Modifications of a commercial perfusion system for use in ultrafast solution exchange during patch clamp recording

    D.J. Hinkle, M.T. Bianchi, R.L. Macdonald

    págs. 472-476

    

  • Isolation protocol for two-dimensional-polyacrylamide gel electrophoreses analysis of Haloferax volcanii proteome

    M. Eschenbrenner, C.V. Mujer, V.G. del Vecchio, E.C. Evans, T. Horn, M.A. Wagner

    págs. 478-482

    

  • Rapid selection of Drosophila S2 cells with the puromycin resistance gene

    F.J. Castellino, T. Iwaki, M. Figuera, V.A. Ploplis

    págs. 482-486

    

  • Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants

    S.D. Lawrence, N.G. Novak, J.M. Slack

    págs. 488-492

    

  • Isolation and purification of functional total RNA from different organs of cacao tree during its interaction with the pathogen Crinipellis perniciosa

    J.C de Mattos Cascardo, A. de Silva Gesteira, F. Micheli, C.F. Ferreira

    págs. 494-500

    

  • Gene-specific monitoring of T7-based RNA amplification by real-time quantitative PCR

    R.H. Finnell, H.J. Blom, S.G. Heil, L.A.J. Kluijtmans, O. Spiegelstein

    págs. 502-508

    

  • Subtraction of cap-trapped full-length cDNA libraries to select rare transcripts

    M. Nakamura, T. Arakawa, J. Kawai, M. Fagiolini, T.K. Hensch, Y. Hayashizaki, P. Carninci, T. Hirozane-Kishikawa, T. Shiraki, K. Waki

    págs. 510-518

    

  • Simultaneous cloning of open reading frames into several different expression vectors

    C.A. Stanyon, T. Limjindaporn, R.L. Finley, jr.

    págs. 520-526

    

  • Comparative study of apoptosis-detecting techniques: TUNEL, apostain, and lamin B

    V. Lichnovsky, J. Prochazkova, D. Kylarova, P. Vranka

    págs. 528-534

    

  • Purification of Mycobacterium leprae RNA for gene expression analysis from leprosy biopsy specimens

    D.M. Scollard, D.L. Williams, S. Oby-Robinson, T.L. Pittman

    págs. 534-541

    

  • Mobility-shift analysis with microfluidics chips

    R. Dabur, L.E. Crocitto, Y.I. Buryanov, S.S. Smith, J. Clark, T. Shevchuk, P.M. Swiderski

    págs. 548-554

    

  • Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers

    S.G. Sligar, N.R. Civjan, T.H. Bayburt, M.A. Schuler

    págs. 556-563

    

  • COMBO-FISH: specific labeling of nondenatured chromatin targets by computer-selected DNA oligonucleotide probe combinations

    J. Finsterle, A. Weisel, A. Rapp, E. Schmitt, S. Janz, C. Cremer, M. Hausmann, R. Winkler, G. Hildenbrand

    págs. 564-577

    

  • BioTechniques Live! 2004 Meeting update

    págs. 578-579

    

  • Integration of genomic technologies for accelerated cancer drug development

    M. Basik, S. Mousses, J. Trent

    págs. 580-593

    

  • Quantitative real-time PCR for gene dosage determinations in microdeletion genotypes

    C. Oncken, J. Covault, C. Abreu, H. Kranzler

    págs. 594-598

    

  • Transfection of murine dendritic cell line (JAWS II) by a nonviral transfection reagent

    S. Awasthi, R.A. Cox

    págs. 600-604

    

  • Automated sequencing of complete mitochondrial genomes from laser-capture microdissected samples

    S. Hosseini, W. Buck, T.L. Almekinder, C.Q. Sun, J.A. Petros, B.A. Aldridge, S.D. Lim, A.K. Baumann

    págs. 606-612

    

  • Analysis of HIV-1 variants by cloning DNA generated with the ViroSeq (TM) HIV-1 Genotyping System

    O. Petrauskene, J.B. Jackson, S.H. Eshleman, D. Jones, T. Flys

    págs. 614-622

    

  • Analysis of apoptotic cells using Beadlyte (R) suspension arrays

    D.P. Eisinger, P.W. Rhyne, J.D. Scull, L.M. Stiles

    págs. 624-629

    

  • New products for bioresearch

    págs. 630-637