BioTechniques

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  Año 1998, Vol. 25, Número 5

  • Generation of RNA Ladders by Rolling Circle Transcription of Small Circular Oligodeoxyribonucleotides

    A. M. Diegelman, S. L. Daubendiek, E. T. Kool

    pág. 754

    

  • Combined PCR/Gapped-Duplex Method for Site-Directed Mutagenesis

    L. Isaac, C. S. Farah

    pág. 758

    

  • Improved Protocol for High-Throughput Cysteine Scanning Mutagenesis

    S. Howorka, H. Bayley

    pág. 764

    

  • Improved Antibiotic-Resistance Cassettes Through Restriction Site Elimination Using Pfu DNA Polymerase PCR

    J. J. Dennis, G. J. Zylstra

    pág. 772

    

  • Uniform Terminus PCR: Amplification of Minute Unknown DNA Fragments

    S. Zhang, C. Xiao, Q. Xia, H. Wu

    pág. 780

    

  • Long-Distance PCR-Based Strategy for Preparing Knock-In Vectors Directly from ES Cell Genomic DNA

    L.-H. Zhang, H. E. Henderson

    pág. 784

    

  • Cryopreserved Yeast Cells Suitable for Routine, Small-Scale Transformations

    S.-L. Tan, M. Dossett, M. G. Katze

    pág. 792

    

  • Extraction of Genomic DNA Suitable for PCR Analysis from Dried Plant Rhizomes/Roots

    P.-C. Shaw, R.-Z. Fu, J. Wang, Y.-R. Sun

    pág. 796

    

  • Improved Reproducibility of ELISA Using a Static-Discharge Pad

    K. J. Cassutt, S. H. Pincus

    pág. 801

    

  • Cyberspace for Biologists

    pág. 804

    

  • Elimination of Residual Natural Nucleotides from 3'-O-Modified-dNTP Syntheses by Enzymatic Mop-Up

    R. A. Gibbs, M. L. Metzker, R. Raghavachari, K. Burgess

    pág. 814

    

  • Comparison of Three RNA Amplification Methods as Sources of DNA for Sequencing

    I. J. Bruce, N. Chadwick, A. J. Wakefield, R. E. Pounder

    pág. 818

    

  • Rapid Hybridoma Screening Method for the Identification of Monoclonal Antibodies to Low-Abundance Cytoplasmic Proteins

    L. O'Connor, D. C. S. Huang, A. Strasser, L. A. O'Reilly, L. Cullen, K. Moriishi

    pág. 824

    

  • Digital Quantification of the Enzyme-Linked Immunospot (ELISPOT)

    Y.-M. Zhou, S. P. L. Leong, J. E. Vaquerano, M. Peng, J.W. Chang

    pág. 830

    

  • Two-Color Green Fluorescent Protein Time-Lapse Imaging

    J. Ellenberg, J. Lippincott-Schwartz, John F. Preseley

    pág. 838

    

  • Dual-Function Reporter Protein for Analysis of Gene Expression in Living Cells

    R. N. Day, M. Kawecki, D. Berry

    pág. 848

    

  • Coherent Backscatter Enhances Reflection Confocal Microscopy

    C. H. Keith, G. J. Bird, M. A. Farmer

    pág. 858

    

  • Cell-Specific Ecdysone-Inducible Expression of FLP Recombinase in Mammalian Cells

    J. A. Sawicki, B. Monks, R. J. Morris

    pág. 868

    

  • PCR-Introduced Loop Structure as Primer in DNA Sequencing

    P. Nyren, M. Ronaghi, B. Pettersson, M. Uhlen

    pág. 876

    

  • Parallel Production of Oligonucleotide Arrays Using Membranes and Reagent Jet Printing

    D. B. Wallace, D. I. Stimpson, P. W. Cooley, S. M. Knepper

    pág. 886

    

  • Reuse of Denaturing Polyacrylamide Gels for Short Tandem Repeat Analysis

    A. Tereba, K. A. Micka, J. W. Schumm

    pág. 892

    

  • Positive Selection Vectors to Generate Fused Genes for the Expression of His-Tagged Proteins

    C. Szpirer, P. Gabant, T. Van Reeth, P.-L. Drèze, J. Szpirer

    pág. 898