BioTechniques

• 

  Año 1999, Vol. 27, Número 3

  • Sequencing of Genomic DNA

    U. Mahlknecht, D. Hoelzer, R. Bucala

    págs. 406-409

    

  • Generation of Full-Length cDNA Library from Single Human Prostate Cancer Cells

    C.-M. Chuong, S.-Y. Ying, H. M. Lui, S.-L. Lin

    págs. 410-413

    

  • Multistep-Touchdown Vectorette-PCR - A Rapid Technique for the Identification of IVS in Genes

    W. Haverkamp, G. Breithardt, C. Rubie, E. Schulze-Bahr, M. Borggrefe

    págs. 414-417

    

  • Rapid, Sensitive Detection of Mutant Alleles in Codon 12 of K-ras by REMS-PCR

    C. J. Fuery, R. L. Ward, A. V. Todd, N. J. Roberts, H. L. Impey, T. L. Applegate

    págs. 418-423

    

  • Male-Specific Competitive PCR for the Quantification of Transplanted Cells in Mice

    N. J. Caron, P. Chapdelaine, J. P. Tremblay

    págs. 424-427

    

  • Construction of a Vector for Coupled In Vitro Transcription/Translation

    O. Ostersetzer, Z. Adam

    págs. 428-434

    

  • Conditions for Permeabilization of Cells Used for Intracellular Tyrosine Phosphorylation Studies

    Z. Hegedus, E. Monostori, V. Chitu, D. Demydenko, G. K. Toth

    págs. 435-437

    

  • Improved Detection Reliability of á-Galactosidase in Histological Preparations

    G. Cheng, R. P. Thompson, R. G. Gourdie

    págs. 438-443

    

  • Nucleic Acid Removal from Taq Polymerase Preparations Using an Aqueous/Organic Biphasic System

    A. Louwrier

    págs. 444-447

    

  • Adaptation of BTI-TN5B1-4 (High Five) Insect Cells for Large-Scale Production in a Stirred Bioreactor

    A.-C. Savary, B. Georges, C. Auriault

    págs. 448-449

    

  • Single-Nucleotide Resolution of RNA Strands in the Presence of their RNA Complements

    C. E. Cameron, J. J. Arnold, S. K. B. Ghosh, P. C. Bevilacqua

    págs. 450-457

    

  • Improved Isolation of Nucleic Acids from Basidiomycete Fungi

    P. Baldrian, J. Gabriel, M. Pospisek

    págs. 458-465

    

  • Plasmid Harboring lac Repressor and tTA Activator Genes Can Regulate Two Inducible Genes in Mammalian Cells

    S.-T. Wang, I.-J. Su, H.-S. Liu, T.-Y. Chang, Y.-Y. Wen, H.-H. Yeh

    págs. 466-469

    

  • An Introduction to Perl: the QuizMaker Revisited

    R. M. Horton

    págs. 470-479

    

  • Construction of Larger-Size Sequencing Templates from Degraded DNA

    I. Nasidze, M. Stoneking

    págs. 480-487

    

  • Recovery of Upstream cDNA Sequences by a PCR-Based Biotin- Capture Method

    M. Uhlen, J. Lundeberg, C. Hoeoeg, S. Stahl, S. Graeslund, M. Larsson, F. Sterky

    págs. 488-499

    

  • Universal Linker and Ligation Procedures for Construction of Genomic DNA Libraries Enriched for Microsatellites

    R. C. Fleischer, M. B. Hamilton, E. L. Pincus, A. Di Fiore

    págs. 500-507

    

  • Template Secondary Structure Promotes Polymerase Jumping During PCR Amplification

    V. K. Viswanathan, K. Krcmarik, N. P. Cianciotto

    págs. 508-511

    

  • Competitive RT-PCR for Measuring Metalloproteinase Gene Expression in Human Mesangial Cells Exposed to a Hyperglycemic Environment

    A. K. Death, D. K. Yue, J. R. Turtle

    págs. 512-519

    

  • Application of In Situ PCR to Yeast Cells for Screening YAC Libraries

    S. Aytay, L. M. Davis, J. C. Hozier

    págs. 520-527

    

  • Design Strategies and Performance of Custom DNA Sequencing Primers

    K. M. Hager, C. W. Naeve, R. T. Pon, P. S. Adams, J. Rush, G. A. Buck, J. W. Fox, M. Gunthorpe

    págs. 528-537

    

  • High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5' Exonuclease Assay

    P. A. Morin, R. Saiz, A. Monjazeb

    págs. 538-553

    

  • Differential Display Probes for cDNA Arrays

    T. Trenkle, J. Welsh, M. McClelland

    págs. 554-565

    

  • Quantitative RT-PCR to Evaluate In Vivo Expression of Multiple Transgenes Using a Common Intron

    M. Lay, S. Corson, E. Fox, C. Luong, G. Koe, B. Lemos, R. Grove, J. Fairman, L. Roche, I. Pieslak

    págs. 566-575

    

  • Introduction of an N-Glycosylation Cassette into Proteins at Random Sites: Expression of Neoglycosylated FGF

    T. Imamura, A. Yoneda, M. Asada, M. Suzuki

    págs. 576-591

    

  • Fabrication of Microarray of Gel-Immobilized Compounds on a Chip by Copolymerization

    A. L. Drobyshev, V. V. Shick, A. D. Mirzabekov, A. V. Vasiliskov, E. N. Timofeev, S. A. Surzhikov

    págs. 592-607

    

  • Tyramide Signal Amplification (TSA)-FISH Applied to Mapping PCR-Labeled Probes Less Than 1 kb in Size

    P. Moen, W. G. Nash, J. Menninger, G. Jones, T. Ried, M. Dean, L. M. Schriml, H. M. Padilla Nash, A. Coleman

    págs. 608-613