Resumen de The use of molecular techniques in the support to conservation of the Chato Murciano breed of pig.
José Luis Vega Plá, María Amparo Martínez Martínez, Begoña Peinado Ramón, Angel Poto Remacha, Juan Vicente Delgado Bermejo
The use of molecular allelic typing tools in small populations near extinction is evaluated. A total of 83 samples of Chato Murciano pig are typed with 26 microsatellites recommended for biodiversity studies. Allelic frequencies are obtained by direct count. Observed, unbiased and expected heterocigosity and pairs of individual distances based in shared alleles are calculated. An individual tree is built from matrix of distances. Allele number is smaller than other pig breeds. The process of breed conservation can be followed by mean of individual phylogenetic tree drawing. Observing, as even it is necesary the creation of a line by using of a LargeWhite female, is shown an absorption of the genotype of this animal in favour of the Chato Murciano genotypes by generations. This approach also facilitates the selection of the best mating.
