Abstract
Purpose
In the present work, we investigated the expression pattern of miR-4463 in the non-metastasis and metastasis colorectal cancer (CRC) patients and its regulation axis.
Methods
RT-qPCR assay was performed to assess miR-4463 expression in the serum and tissues of patients with non-metastasis and metastasis, and in the CRC cell lines. MTT assay, colony formation assay, transwell assay, and flow cytometry assay were used to examine the role of miR-4463 in CRC cell viability, proliferation, and migration. Bioinformatic analysis was used to identify the potential target gene of miR-4463, and the targeting relationship between miR-4463 and PPP1R12B was verified in vitro using dual luciferase assay. Western blotting assay was used to determine the protein level of the target gene PPP1R12B in CRC cells under the transfections of miR-4463 mimic, inhibitor and vectors overexpressing PPP1R12B.
Results
miR-4463 was markedly increased in the non-metastasis CRC tissues, and increased even higher in the metastasis CRC tissues, while miR-4463 expression had no significant difference in serum from non-metastasis and metastasis CRC samples. Besides, miR-4463 was upregulated in CRC cell lines. Functionally, miR-4463 promoted CRC cell proliferation, migration, and inhibiting cell apoptosis. Further analysis revealed that the miR-4463/PPP1R12B axis was responsible for the role of this miRNA.
Conclusion
We reported the roles of miR-4463 in CRC proliferation and migration, supporting that miR-4463 could be a potential predictive diagnostic marker for colon cancer.
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We would like to thank all the researchers and study participants for their contributions.
Funding
This study was supported by the mechanism and expression of miR-4463 in patients with colorectal cancer (Project label: GCPY201710); A detection kit of miRNA for early diagnosis of colorectal cancer (Project label: ZHZD201801); Zhu Yong (QRX17092); Academic leader and talent cultivation (Project label: YRC2014-ZY3).
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JT, BJ, YZ and TL conceived the study. JT, TL and JX wrote the manuscript and designed the figures. YH, ZC, KZ and YD contributed to the writing and editing of the manuscript. All authors and participants reviewed the paper and approved the final manuscript.
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The study was approved by Ethical Committee of The Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine and conducted in accordance with the ethical standards.
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Tan, J., Lu, T., Xu, J. et al. MicroRNA-4463 facilitates the development of colon cancer by suppression of the expression of PPP1R12B. Clin Transl Oncol 24, 1115–1123 (2022). https://doi.org/10.1007/s12094-021-02752-0
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DOI: https://doi.org/10.1007/s12094-021-02752-0