Abstract
Purpose
Increasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. Therefore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS.
Methods
RT-qPCR experiment was used to detect miR-802 and p27 expression in OS tissues and cells. We explored the function of miR-802 through Transwell assays. The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelial–mesenchymal transition (EMT) was detected by Western blot assays. Luciferase assay was used to testify the target of miR-802.
Results
MiR-802 expression was elevated in OS, which was related to poor clinical outcome in OS patients. MiR-802 overexpression promoted OS migration, invasion and EMT. Further, p27 is a direct target of miR-802. P27 elevation counteracted the promotion effect of OS on EMT, migration and invasion induced by miR-802. In addition, miR-802 overexpression inactivated PI3K/AKT pathway via targeting p27 in OS.
Conclusion
MiR-802 promoted the progress of EMT, migration and invasion in OS via targeting p27. This newly identified miR-802/p27/PI3K/AKT axis may represent potential targets for OS.
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LG conceived and designed the study and drafted the manuscript. SJ and QZ collected, analyzed and interpreted the experimental data. YX, CL and YL revised the manuscript for important intellectual content. All authors read and approved the final manuscript.
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The study was approved by Ethical Committee of Weifang Weiyi Tumor Hospital and conducted in accordance with the ethical standards. Approval No. 2012-11.
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Gao, .F., Jia, S., Zhang, .M. et al. MicroRNA-802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway. Clin Transl Oncol 24, 266–275 (2022). https://doi.org/10.1007/s12094-021-02683-w
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DOI: https://doi.org/10.1007/s12094-021-02683-w