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Modulation of intestinal L-glutamate transport by luminal leptin

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Abstract

Leptin is secreted into the digestive tract and contributes to the absorption of dietary molecules by regulating transporters activity. Here, we studied the effect of luminal leptin on the intestinal transport of L-glutamate, an important component of human diet. We examined the effect of leptin on L-glutamate uptake in rat intestine in vitro measuring glutamate-induced short-circuit current (Isc) in Ussing chambers and L-[3H (U)]-glutamate uptake in jejunal everted rings. Glutamate-induced Isc was only observed in Na+-free conditions. This Isc was concentration (1–60 mmol L−1) and pH dependent. Luminal leptin increased glutamate Isc (∼100 %). Dose-response curve showed a biphasic pattern, with maximal stimulations observed at 10−13 and 10−10 mmol L−1, that were sensitive to leptin receptor antagonist. In everted rings, two glutamate transport mechanisms were distinguished: a Na+-dependent, H+-independent, that was inhibited by leptin (∼20 %), and a Na+-independent but H+-dependent, that was enhanced by leptin (∼20 %), in line with data obtained in Ussing chambers. Altogether, these data reveal original non-monotonic effect of luminal leptin in the intestine and demonstrate a new role for this hormone in the modulation of L-glutamate transport, showing that luminal active gut peptides can influence absorption of amino acids.

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Acknowledgments

We thank Asunción Redín for her unconditional technical assistance; Ana Barber, Maude Le Gall, and Katia Marazova for their helpful comments.

This work was supported by the “Ministerio de Educación y Ciencia”, Spain (Grant BFU2007-60420/BFI) and the “Fundación Marcelino Botín”. The Spanish group is member of the Network for Cooperative Research on Membrane Transport Proteins (REIT), co-funded by the “Ministerio de Educación y Ciencia”, Spain and the European Regional Development Fund (ERDF) (Grant BFU2007-30688-E/BFI). C. Fanjul has been a recipient of a pre-doctoral fellowship from the “Asociación de Amigos”, University of Navarra.

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No conflicts of interest, financial or otherwise, are declared by the authors.

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    Authors and Affiliations

    1. Department of Nutrition, Food Science and Physiology, School of Pharmacy, University of Navarra, Pamplona, Spain

      Carmen Fanjul, Jaione Barrenetxe & María Pilar Lostao

    2. Institut National de la Santé et de la Recherche Médicale, UMRS1149, Faculté de Médicine Xavier Bichat, Paris, France

      Robert Ducroc

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    1. Carmen Fanjul
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    2. Jaione Barrenetxe
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    Correspondence to María Pilar Lostao.

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    María Pilar Lostao and Robert Ducroc contributed equally to this work.

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    Fanjul, C., Barrenetxe, J., Lostao, M.P. et al. Modulation of intestinal L-glutamate transport by luminal leptin. J Physiol Biochem 71, 311–317 (2015). https://doi.org/10.1007/s13105-015-0414-z

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