Abstract
Calcium is an essential nutrient required for critical biological functions. Calcium supplementation is to be evaluated using immature female rats. The present study focused on some blood parameters, gonadal development and bone structure. Forty immature female Sprague–Dawley rats were randomly divided into four equal-sized groups (80 g average body weight) to receive calcium chloride dihydrate (group I: control; groups II, III and IV: received 20 mg, 40 mg and 60 mg per kg body weight, respectively) for 5 weeks. Rats were decapitated, and their trunk blood was sampled for biochemical assays. Cholesterol, triglycerides, glucose and calcium were measured. Gonadal and bone structure were histologically evaluated. Results revealed that treatment of developing female rats with three calcium doses used have no marked effect on the serum calcium and cholesterol levels. However, serum triglyceride level and body weight gain are significantly decreased in rats treated with all of the three calcium doses. Serum glucose level showed a marked increase in animals treated with the higher calcium doses. Moreover, observable histological alterations are recognized in the ovaries. Bones of the experimental animals also showed morphological alterations. These results suggest that increasing calcium supplementation decreases triglycerides and percentage body weight gain and positively affects the bone and gonadal development.
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Boer I, Tinker L, Connelly S, Curb J, Howard B, Kestenbaum B (2008) Calcium plus vitamin D supplementation and the risk of incident diabetes in the Women's Health Initiative. Diabetes Care 31:701–707
Boon N, Koppes LL, Saris WH, Van Mechelen W (2005) The relation between calcium intake and body composition in a Dutch population: the Amsterdam Growth and Health Longitudinal Study. Am J Epidemiol 162:27–32
Bostick RM, Fosdick L, Grandits GA, Grambsch P, Gross M, Louis TA (2000) Effect of calcium supplementation on serum cholesterol and blood pressure. Arch Fam Med 9:31–39
Caan B, Neuhouser M, Aragaki A, Lewis CB, Jackson R, LeBoff MS, Margolis KL, Powell L, Uwaifo G, Whitlock E, Wylie-Rosett J, LaCroix A (2007) Calcium plus vitamin D supplementation and the risk of postmenopausal weight gain. Arch Intern Med 167:893–902
Cadogan J, Eastell R, Jones N, Barke ME (1997) Milk intake and bone mineral acquisition in adolescent girls: randomised, controlled intervention trial. British Medical Journal 315:1255–1260
Chan GM, Hoffman K, McMurray M (1995) Effects of dairy products on bone and body composition in pubertal girls. J Pediatr 126:551–556
Chevalley T, Rizzoli R, Hans D, Ferrari S, Bonjour JP (2005) Interaction between calcium intake and menarcheal age on bone mass gain: an eight-year follow-up study from prepuberty to postmenarche. J Clin Endocrinol Metab 90:44–51
Cummings NK, James AP, Soares MJ (2006) The acute effects of different sources of dietary calcium on postprandial energy metabolism. Br J Nutr 96:138–44
Curtiss H, LuAnn J (2007) Calcium requirements: new estimations for men and women by cross-sectional statistical analyses of calcium balance data from metabolic studies. American Journal of Clinical Nutr 86:1054–1063
Davies KM, Heaney RP, Recker RR, Lappe JM, Barger-Lux MJ, Rafferty K, Hinders S (2000) Calcium intake and body weight. J Clin Endocrinol Metab 85:4635–4638
De Jongh ED, Binkley TL, Specker BL (2006) Fat mass gain is lower in calcium-supplemented than in unsupplemented preschool children with low dietary calcium intakes. Am J Clin Nutr 84:1123–1127
Denke MA, Fox MM, Schulte MC (1993) Short-term dietary calcium fortification increases fecal saturated fat content and reduces serum lipids in men. J Nutr 123:1047–1053
Dibba B, Prentice A, Ceesay M, Mendy M, Darboe S, Stirling DM, Cole TJ, Poskitt EM (2002) Bone mineral contents and plasma osteocalcin concentrations of Gambian children 12 and 24 mo after the withdrawal of a calcium supplement. Am J Clin Nutr 76:681–686
Dibba B, Prentice A, Poskitt EME, Cole TJ (1998) Calcium supplementation increases the bone mineral status of Gambian children. Proc Nutr Soc 57:73A
Flynn A, Cashman K (1999) Calcium. In: Hurrel R (ed) The mineral fortification of foods. Leatherhead, Surrey, pp 18–53
Fumeron F, Lamri A, Abi Khalil C, Jaziri R, Porchay-Balderelli I (2011) Dairy consumption and the incidence of hyperglycemia and the metabolic syndrome, results from a French prospective study, data from the Epidemiological Study on the Insulin Resistance Syndrome (DESIR). Diabetes Care 34:813–817
Greer FR, Krebs NF (2006) Optimizing bone health and calcium intakes of infants, children, and adolescents. Pediatrics 117:578–585
Heaney RP (2000) Calcium, dairy products and osteoporosis. J Am Coll Nutr 19:83S–99S
Hsu HH, Culley NC (2006) Effects of dietary calcium on atherosclerosis, aortic calcification and icterus in rabbits fed a supplemental cholesterol diet. Lipids Health Dis 5:16
Isaia G, Giorgino R, Adami S (2001) High prevalence of hypovitaminosis D in female type 2 diabetic population. Diabetes Care 24:1496
Jacqmain M, Doucet E, Després JP, Bouchard C, Tremblay A (2003) Calcium intake, body composition, and lipoprotein–lipid concentrations in adults. Am J Clin Nutr 77:1448–1452
Johnson L, DeLuca H (2001) Vitamin D receptor null mutant mice fed high levels of calcium are fertile. American Society for Nutritional Sciences J Nutr 131:1787–1791
Johnston CC, Miller JZ, Slemenda CW, Reister TK, Hui S, Christian JC, Peacock M (1992) Calcium supplementation and increases in bone mineral density in children. N Engl J Med 327:82–87
Jolma P, Kööbi P, Kalliovalkama J, Kähönen M, Fan M, Saha H, Helin H, Lehtimäki T, Pörsti I (2003) Increased calcium intake reduces plasma cholesterol and improves vasorelaxation in experimental renal failure. Am J Physiol Heart Circ Physiol 285:H1882–H1889
Karandish M, Shockravi S, Jalali MT, Haghighizadeh MH (2009) Effect of calcium supplementation on lipid profile in overweight or obese Iranian women: a double-blind randomized clinical trial. Eur J Clin Nutr 63:268–272
Ht L, Eastell R, Karnik K, Russell J, Margo E (2008) Calcium supplementation and bone mineral accretion in adolescent girls: an 18-mo randomized controlled trial with 2-y follow-up. Barker Am J Clin Nutr 87:455–462
Lee WT, Leung SS, Leung DM, Wang SH, Xu YC, Zeng WP, Cheng JC (1997) Bone mineral acquisition in low calcium intake children following the withdrawal of calcium supplement. Acta Paediatr 86:570–576
Lee WT, Leung SS, Wang SH, Xu YC, Zeng WP, Lau J, Oppenheimer SJ, Cheng JC (1994) Double-blind, controlled calcium supplementation and bone mineral accretion in children accustomed to a low-calcium diet. Am J Clin Nutr 60:744–775
Lloyd T, Andon MB, Rollings N, Martel JK, Landis JR, Demers LM, Eggli DF, Kieselhorst K, Kulin HE (1993) Calcium supplementation and bone mineral density in adolescent girls. JAMA 270:841–844
Loos RJ, Rankinen T, Leon AS, Skinner JS, Wilmore JH, Rao DC, Bouchard C (2004) Calcium intake is associated with adiposity in black and white men and white women of the HERITAGE Family Study. J Nutr 134:1772–1778
Lorenzen JK, Mølgaard C, Michaelsen KF, Astrup A (2006) Calcium supplementation for 1 y does not reduce body weight or fat mass in young girls. Am J Clin Nutr 83:18–23
Lorenzen JK, Nielsen S, Holst JJ, Tetens I, Rehfeld JF, Astrup A (2007) Effect of dairy calcium or supplementary calcium intake on postprandial fat metabolism, appetite, and subsequent energy intake. Am J Clin Nutr 85:678–687
Major GC, Alarie F, Dore J, Phouttama S, Tremblay A (2007) Supplementation with calcium + vitamin D enhances the beneficial effect of weight loss on plasma lipid and lipoprotein concentrations. Am J Clin Nutr 85:54–59
Malekzadeh J, Keshavarz A, Siassi F, Kadkhodaei M, Eshraghian M, Dorosti-Motlagh A, Aliehpoor A, Chamari M (2007) Effect of dietary calcium on concentrations of lipids, glucose and insulin in male Sprague–Dawely rats. RYA Ather 3:14–20
Melanson EL, Donahoo WT, Dong F, Ida T, Zemel MB (2005) Effect of low- and high-calcium dairy-based diets on macronutrient oxidation in humans. Obes Res 13:2102–12
Miller G, Jarvis J, McBean L (2001) The importance of meeting calcium needs with foods. J Am Coll Nutr 20:168S–185S
Milner RD, Hales CN (1967) The role of calcium and magnesium in insulin secretion from rabbit pancreas studied in vitro. Diabetologia 3:47–49
Moore LL, Bradlee ML, Gao D, Singer MR (2006) Low dairy intake in early childhood predicts excess body fat gain. Obesity (Silver Spring) 14:1010–1018
Mullender MG, Huiskes R, Versleyen H, Buma H (2005) Osteocyte density and histomorphometric parameters in cancellous bone of the proximal femur in five mammalian species. J Orhtop Res 14(6):972–979
NIH Consensus Development Panel (1994) Optimal calcium intake. JAMA 272:1942–1948
Orwoll E, Riddle M, Prince M (1994) Effects of vitamin D on insulin and glucagon secretion in non-insulin-dependent diabetes mellitus. Am J Clin Nutr 59:1083–1087
Papakonstantinou E, Flatt WP, Huth PJ, Hrris R (2003) High dietary calcium reduces body fat content, digestibility of fat, and serum vitamin D in rats. Obesity Res 11:387–394
Peterson CA, Eurell JA, Erdman JW (1995) Alterations in calcium intake on peak bone mass in the female rat. J Bone Miner Res 10:81–95
Pittas AG, Dawson-Hughes B, Li T, Van Dam RM, Willett WC, Manson JE, Hu FB (2006) Vitamin D and calcium intake in relation to type 2 diabetes in women. Diabetes Care 29:650–656
Pittas AG, Harris SS, Stark PC, Dawson-Hughes B (2007) The effects of calcium and vitamin D supplementation on blood glucose and markers of inflammation in nondiabetic adults. Diabetes Care 30:980–986
Porthouse J, Cockayne S, King C, Saxon L, Steele E, Aspray T, Baverstock M, Birks Y, Dumville J, Francis R, Iglesias C, Puffer S, Sutcliffe A, Watt I, Torgerson DJ (2005) Randomised controlled trial of calcium and supplementation with cholecalciferol (vitamin D3) for prevention of fractures in primary care. BMJ 330:1003
Reid IR, Horne A, Mason B, Ames R, Bava U, Gamble GD (2005) Effects of calcium supplementation on body weight and blood pressure in normal older women: a randomized controlled trial. J Clin Endocrinol Metab 90:3824–3829
Shahkhalili Y, Murset C, Meirim I, Duruz E, Guinchard S, Cavadini C, Acheson K (2001) Calcium supplementation of chocolate: effect on cocoa butter digestibility and blood lipids in humans. Am J Clin Nutr 73:246–252
Shi H, Dirienzo D, Zemel MB (2001) Effects of dietary calcium on adipocyte lipid metabolism and body weight regulation in energy-restricted aP2-agouti transgenic mice. FASEB J 15:291–293
Shi H, Norman AW, Okamura WH, Sen A, Zemel MB (2001) 1alpha,25-Dihydroxyvitamin D3 modulates human adipocyte metabolism via nongenomic action. FASEB J 15:2751–2753
Slemenda CW, Peacock M, Hui S, Zhou L, Johnston CC (1997) Reduced rates of skeletal remodeling are associated with increased peak bone mineral density during the development of peak skeletal mass. J Bone Miner Res 12:676–682
Sun C, Yu X, LI Y, Liu R (2004) Effects of dietary calcium on the blood glucose, blood lipid and hormone of rat fed a high fat diet. Wei Sheng Yan Jiu 33:164–166
Talbott SM, Chowdhury H, Shapses SA (1999) Urinary 3H-tetracycline and pyridinium crosslinks differ in their response to calcium restriction in mature and aged rats. Calcif Tissue Int 64:352–356
Tang BM, Eslick GD, Nowson C, Smith C, Bensoussan A (2007) Use of calcium or calcium in combination with vitamin D supplementation to prevent fractures and bone loss in people aged 50 years and older: a meta-analysis. Lancet 370:657–666
Trowman R, Dumville JC, Hahn S, Torgerson DJ (2006) A systematic review of the effects of calcium supplementation on body weight. Br J Nutr 95:1033–1038
Van Dam RM, Hu FB, Rosenberg L, Krishnan S, Palmer JR (2006) Dietary calcium and magnesium, major food sources, and risk of type 2 diabetes in U.S. black women. Diabetes Care 29:2238–2243
Vaskonen T (2003) Dietary minerals and modification of cardiovascular risk factors. J Nutr Biochem 14:492–506
Vaskonen T, Mervaala E, Sumuvuori V, Seppanen-Laakso T, Karppanen H (2002) Effects of calcium and plant sterols on serum lipids in obese Zucker rats on a low-fat diet. Br J Nutr 87:239–245
Welberg JW, Monkelbaan JF, de Vries EG, Muskiet FA, Cats A, Oremus ET, Boersma-van Ek W, van Rijsbergen H, van der Meer R, Mulder NH (1994) Effects of supplemental dietary calcium on quantitative and qualitative fecal fat excretion in man. Ann Nutr Metab 38:185–191
Williams PF, Caterson ID, Cooney GJ, Zilkens RR, Turtle JR (1990) High affinity insulin binding and insulin receptor–effector coupling: modulation by Ca21. Cell Calcium 11:547–556
Winzenberg T, Shaw K, Fryer J, Jones G (2007) Calcium supplements in healthy children do not affect weight gain, height, or body composition. Obesity (Silver Spring) 15:1789–1798
Yin J, Zhang Q, Liu A, Du W, Wang X, Hu X, Ma G (2010) Calcium supplementation for 2 years improves bone mineral accretion and lean body mass in Chinese adolescents. National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention. Beijing, China Asia Pac J Clin Nutr 19(2):152–160
Zemel MB (2004) Role of calcium and dairy products in energy partitioning and weight management. Am J Clin Nutr 79:907S–912S
Zemel MB, Miller SL (2004) Dietary calcium and dairy modulation of adiposity and obesity risk. Nutr Rev 62:125–131
Zemel MB, Shi H, Greer B, Dirienzo D, Zemel PC (2000) Regulation of adiposity by dietary calcium. FASEB J 14:1132–1138
Zhang Q, Tordoff MG (2004) No effects of dietary calcium on body weight of lean and obese mice and rats. Am J Physiol Regul Integr Comp Physiol 286:R669–R677
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The authors thank the technical staff of the Department of Biological and Environmental Sciences, Faculty of Science, Beirut Arab University, Lebanon for their helpful assistance.
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El-Merhie, N., Sabry, I. & Balbaa, M. Effect of calcium treatment on blood parameters, gonadal development and the structure of bone in immature female rats. J Physiol Biochem 68, 219–227 (2012). https://doi.org/10.1007/s13105-011-0133-z
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DOI: https://doi.org/10.1007/s13105-011-0133-z