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Regulation of adiponectin gene expression in adipose tissue by thyroid hormones

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Abstract

Available experimental data suggest that adiponectin and thyroid hormones have biological interaction in vivo. However, the effects of thyroid hormones on adipose adiponectin gene expression in thyroid dysfunction are unclear. We induced hyper- (HYPER) and hypothyroidism (HYPO) by daily administration of a 12 mg/l of levothyroxine and 250 mg/l of methimazole in drinking water of rats, respectively, for 42 days. The white adipose tissues and serum sample were taken on days 15, 28, 42 and also 2 weeks after treatment cessation. Analysis of adiponectin gene expression was performed by real-time PCR and 2−ΔΔct method. The levels of adipose tissue adiponectin mRNA in the HYPO rats were decreased during the 6-week treatment when compared to control rats (<0.05) and were increased significantly 2 weeks after HYPO cessation (P < 0.05). This decline in adiponectin gene expression occurred in parallel with a decrease in T3, T4, fT3 and fT4 concentrations (P < 0.05). In opposite to HYPO rats, adipose adiponectin gene expression was increased in HYPER rats during the 6-week treatment in parallel with an increase the thyroid hormones concentrations (P < 0.05), and its expression was decreased 2 weeks after HYPER cessation (P < 0.05). Adiponectin gene expression levels showed significant negative correlations with concentrations of LDL (HYPO; r = −0.806, P = 0.001 and HYPER; r = −0.749, P = 0.002), triglyceride (HYPO; r = −0.825, P = 0.001 and HYPER; r = −0.824, P = 0.001) and significant positive correlations with concentrations of glucose (HYPO; r = 0.674, P = 0.004 and HYPER; r = 0.866, P = 0.001) and HDL (HYPO; r = 0.755, P = 0.001 and HYPER; r = 0.839, P = 0.001). The current study provides evidence that adiponectin gene expression in adipose tissue is regulated by thyroid hormones at the translation level and that lipid and carbohydrate disturbances in a patient with thyroid dysfunction may be, in part, due to adiponectin gene expression changes.

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Acknowledgment

This study was financially supported by the Shahid Chamran University of Ahvaz (Grant No. 89/3/02/44305) and Shiraz Unuversity.

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Authors and Affiliations

  1. Department of Biochemistry, Research and Science Branch, Islamic Azad University, Tehran, Iran

    Samira Seifi

  2. Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mohammad Reza Tabandeh & Parisa Sarkoohi

  3. Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Saed Nazifi

  4. Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Mehdi Saeb

  5. Department of Phatobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Sadegh Shirian

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  1. Samira Seifi
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  2. Mohammad Reza Tabandeh
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Correspondence to Mohammad Reza Tabandeh.

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Seifi, S., Tabandeh, M.R., Nazifi, S. et al. Regulation of adiponectin gene expression in adipose tissue by thyroid hormones. J Physiol Biochem 68, 193–203 (2012). https://doi.org/10.1007/s13105-011-0131-1

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  • DOI: https://doi.org/10.1007/s13105-011-0131-1

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