Epigenetic regulation of the transcriptome
- Autores: Beatrice Borsari
- Directores de la Tesis: Roderic Guigó Serra (dir. tes.)
- Lectura: En la Universitat Pompeu Fabra ( España ) en 2021
- Idioma: español
- Tribunal Calificador de la Tesis: Thomas R. Gingeras (presid.), Renée Beekman (secret.), Mark Gerstein (voc.)
- Programa de doctorado: Programa de Doctorado en Biomedicina por la Universidad Pompeu Fabra
- Materias:
- Enlaces
- Tesis en acceso abierto en: TDX
- Resumen
We have monitored the transcriptome and the epigenome of human pre-B cells transdifferentiating into macrophages. Analysis of these data provides a general framework to understand the relationship between gene expression and chromatin. We have observed widespread uncoupling of gene expression and epigenetic features during transdifferentiation, with several genes characterized by unvaried chromatin state throughout the process, irrespective of changes in gene expression. Nevertheless, we report a strong association between transcription and chromatin marking of promoter regions at the time of initial gene activation. We have also analyzed the genomic location of distal regulatory elements in developmental and adult samples, and found that tissue-specific enhancer signatures in the human genome tend to accumulate within introns, while those shared among tissues are more frequently intergenic. By focusing on intronic segments, we have additionally uncovered both constraint and variation in the timing of splicing, with a subset of introns that switch from co-transcriptional to post-transcriptional splicing across distinct cell types.
