“chimeric synthetic peptides as diagnostic tools. A novel igm-specific immunoassay for the diagnosis of toxoplasmosis”,
- Autores: Greta Ripoll Pastor
- Directores de la Tesis: Roser Llevadot Esquerda (dir. tes.), David Andreu Martínez (dir. tes.)
- Lectura: En la Universitat Pompeu Fabra ( España ) en 2021
- Idioma: español
- Materias:
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- Resumen
Serologic tests detecting anti-T. gondii IgMs are the gold standard for the diagnosis of acute toxoplasmosis. Nevertheless, most commercial kits are based on lysate antigens obtained from tachyzoites grown in mice or tissue culture which present inherent limitations in terms of performance, providing erroneous results to the physicians, thus hindering patient management and requiring stringent regulation.
In this context, peptide-based antigens have emerged as an attractive alternative to overcome several of such issues. In this thesis, we focused on designing and synthetizing a novel T. gondii IgM-specific chimeric peptide that was used to develop a chemiluminescent immunoassay for the detection of anti-T. gondii IgMs. Additionally, we aimed to assess mouse monoclonal humanized chimeric antibodies as an alternative source to the human positive plasma-derived samples that are currently used to produce reference materials, such as calibrators and controls.
Along with its specific results, this thesis provides a set of innovative technologies to discover IgMspecific sequences translating them into robust antigens, to optimize immunoassay formulations, and to replace the current supply-chain of reference materials.
