Serological detection of Trichinella spiralis and Trichinella britovi in wild boar by ELISA using an excretor-secretor antigen and a crude antigen

• Autores: José Antonio Gamito Santos, Juan Blanco Ciudad, Isidro Suárez López, Juan Enrique Pérez Martín, Francisco Javier Serrano Aguilera
• Localización: Revista Ibero-latinoamericana de parasitología, ISSN 0718-8730, Vol. 70, Nº. 1, 2011, págs. 49-57
• Idioma: inglés
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• Resumen

  • The enzyme-linked immunosorbent assay (ELISA) method is extensively used in epidemiological studies in order to establish Trichinella negligible areas. In this study, we examined the sensitivity obtained by the ELISA method using different antigens and the two Trichinella species found in Spain. We analyzed the humoral evolution that is produced with the Excretor-Secretor (ES) and Crude Larval (CWE) antigens, in two species of Trichinella. The results confi rm greater precocity and persistence of antibodies when faced with higher infective doses and better detection of Trichinella spp. when using the antigen homologous to the infected species. The sensitivity obtained with ELISA is at least equal to that of digestion and greater if Trichinella britovi antigens are used. We have demonstrated a distinct kinetic pattern in the two species of Trichinella studied. Our results support the usefulness of this method in epidemiological surveillance and its optimization for the use as a serological test in the detection of this zoonosis.