Validated stability indicating rp-hplc method for simultaneous determination of nortriptyline and pregabalin in bulk and combined dosage formulations

• HARITHA POTLURI ^[2] ; SREENIVASA RAO BATTULA ^[1] ; SUNANDAMMA YETURU ^[3]
  1. [1] GITAM University

     GITAM University

     India

     
  2. [2] JNTUK Department of Chemistry
  3. [3] Acharya Nagarjuna University Department of Chemistry

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• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 62, Nº. 2, 2017, págs. 3490-3495
• Idioma: inglés
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  • A simple and precise stability indicating RP-HPLC method was developed for simultaneous analysis of drug nortriptyline and pregabalin using BDS (250mm x 4.6 mm, 5μ) C18 column at 210 nm of UV detection. Perchloric acid (0.1%) and acetonitrile in the ratio of 55:45 was used as the mobile phase with a flow rate of 1.0 ml/min and linearity response was established over the concentration range of 5-30 μg/ml for nortriptyline and 37.5-225 μg/ml for pregabalin. The active pharmaceutical ingredients recovered for nortriptyline and pregabalin are in the range of 100.60-101.65% and 100.59-101.74% respectively. The method was validated and was found to be stability indicating and can be successfully utilized for the quantitative analysis of pharmaceutical tablet dosage formulations containing nortriptyline and pregabalin.