Resumen de Nanosuspensions as carriers of Annona muricata acetogenins: antibacterial activity against Enterococcus faecalis and listeria monocytogenes
Brandon Alexis López Romero, Guillermo Téllez Isaías, Gabriel Luna Bárcenas, Adalberto Zamudio Ojeda, Gilberto Velázquez Juárez, María de Lourdes García Magaña, Gabriela Aguilar Hernández, Efigenia Montalvo González
This research aimed to develop nanosuspensions as carriers of Annona muricata acetogenins (ACGs) using different concentrations of soy lecithin (SL), hydroxypropyl-β-cyclodextrin (βCD) and the dispersion method (low agitation, middle agitation and ultrasound) on particle size and polydispersity index (PDI). A response surface analysis was performed to find an optimized, validated and characterized nanosuspension (OβCDSL-ACGs-NSps) and to measure its antibacterial activity. The best conditions to formulate OβCDSL-ACGs-NSps with spherical nanoparticles (133.77 nm), PDI (0.20) and good stability (−37 mV) were 10 mg/mL SL, 0.16% βCD and ultrasound as a dispersion method. Nanoparticles from OβCDSL-ACGs-NSps exhibited a high entrapment (85.33%) and encapsulation efficiency (56.09%). The highest release (84.15%) of ACGs was observed until 36 h. OβCDSL-ACGs-NSps also showed significant antibacterial inhibition against E. faecalis (87%) and L. monocytogenes (75%) at 320 µg/mL. βCDSL-ACGs-NSps is an attractive and promising alternative to improve the solubility and bioavailability of ACGs in pharmaceutical applications.
