Resumen de Correlación entre el test de endosmosis (HOST) y otros parámetros de calidad espermática en semen criopreservado de búfalos Murrah
A. Quintero Moreno, C. Osorio Meléndez, D. González Bermejo, H. Nava, J. Rubio Guillén
The present study was conduced to determine the correlation of Hypo-osmotic swelling test (HOST) with routine sperm evaluation parameters of frozen-thawed semen (FTS) of Murrah buffalos. Percentages of sperm vitality (PV), acrosomal integrity (PAI), progressive motility (PMP) and rapid (PER)/ static (PEE) sperm associated with HOST positive sperm percentage were evaluated in 15 straws of 5 buffalo bulls. One part of FTS was used for the evaluation of sperm motility with Sperm Class analyzer®, while the other part was centrifugated and diluted with Phosphate buffered saline (PBS) to use in the evaluation of others parameters, and HOST preparation (osmolarity: 102 mOsm/L). Statistical analysis revealed a significant correlation between PER (positve, p<0.01), PEE (negative, p<0.01), PV (positive, p<0.05) and percentage of HOST. It was inferred that HOST could be a valuable method for routine evaluation of FTS.
