Resumen de Fermentación in vitro de pepsina/pancreatina con inóculo de conejos
R. Abad Guamán, M.D. Carro, J.A. Larrea Dávalos, J. Vanegas, Cesar Enrique Ocasio Vega, R. Carabaño, J. García
The in vitro gas production of pepsin/pancreatin used in the determination of in vitro digestibility was evaluated using different inocula from rabbits (ileal, caecal and soft faeces from rabbits). In experiment 1 were used 3 different ileal and caecal inocula obtained each one from the combination of the digesta of 3 different 70 d old rabbits. In experiment 2 were used 8 inocula (soft faeces) from 8 rabbits (40 d old). In both experiments were added to each bottle 12.5 g of pepsin and 50 g of pancreatin and was compared with others in which no pepsin/pancreatin was added. Gas production curves were adjusted to an empiric sigmoid model. Both ileal and caecal microbiota showed a high capacity to ferment pepsin/pancreatin, as it was observed from the difference asymptotic gas production with and without pepsin/pancreatin (54 vs. 16 ml/bottle; P<0.001) and their lower final pH (6.49 vs. 7.74; P<0.001). No difference between ileal and caecal inocula was observed (P>0.50).Time necessary to reach the asymptotic gas production was short (2×B <13 h). The difference in the asymptotic gas production was also observed when soft faeces were used as inocula (15.4 vs. 4.85 ml; P<0.001). In vitro fermentation with pepsin/pancreatin rendered threefold the amount of gas than without them, independently of the type of inocula, and confirms the capacity of intestinal microbiota to ferment this fraction even
