Resumen de Influence of Body Fat on Oxidative Stress and Telomere Length of Master Athletes

Samuel Silva Aguiar, Thiago S. Rosa, Caio Victor Sousa, Patrick A. Santos, Lucas Barbosa Pelissari, Lysleine Alves Deus, Erica C. Rosa, Rosangela V. Andrade, Herbert Gustavo Simões

• The present investigation analyzed the role of body fat and training history on biological aging of master athletes by comparing and verifying the relationships between markers of adiposity, oxidative balance, and telomere length (TL) in middle-aged runners and untrained individuals. Master athletes (sprinters and endurance runners, n = 21; 51.62 +/- 8.19 years) and untrained age-matched controls (n = 11; 45.41 +/- 10.34 years) had blood samples collected for biochemical and biomolecular analyzes. Pro-oxidant and antioxidant measures as well as DNA extraction were performed using commercial kits. Relative TL (T/S) was determined in leukocytes through quantitative polymerase chain reaction analyses. Master athletes had lower body fat and longer TL than untrained controls (body fat: 12.21 +/- 4.14% vs. 26.03 +/- 4.29%; TL: 1.10 +/- 0.84 vs. 0.56 +/- 0.56 T/S; p < 0.05). Furthermore, master athletes also showed a better oxidative balance than untrained controls (p < 0.05). A negative correlation was observed between TL and body fat (r = -0.471; p = 0.007), and conicity index (r = -0.407; p = 0.021), catalase activity (r = -0.569; p = 0.001), and CAT/TBARS ratio (r = -0.463; p = 0.008) for the whole sample. In conclusion, master athletes have longer TL, better oxidative profile, and lower body fat than untrained individuals. Moreover, for this middle-aged sample, body fat was inversely correlated with both TL and markers of oxidative balance, demonstrating the key role of adiposity in biological aging.