Enhanced anticancer activity and endocytic mechanisms by polymeric nanocarriers of n‑butylidenephthalide in leukemia cells

• X.F. Huang ^[1] ; P.T. Chen ^[1] ; Y.L. Lin ^[3] ; M.S. Lee ^[1] ; K.F. Chang ^[1] ; K.W. Liao ^[2] ; G.T. Sheu ^[1] ; M.C. Hsieh ^[1] ; N.M. Tsai ^[1]
  1. [1] Chung Shan Medical University

     Chung Shan Medical University

     Taiwán

     
  2. [2] National Chiao Tung University

     National Chiao Tung University

     Taiwán

     
  3. [3] Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan, ROC

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• Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 23, Nº. 6, 2021, págs. 1142-1151
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • PurposeThe purpose of this study was to investigate the antitumor mechanisms of n-butylidenephthalide (BP) and to further examine the delivery efficacy of polycationic liposome containing PEI and polyethylene glycol complex (LPPC)-encapsulated BP in leukemia cells.MethodsMTS, flow cytometric and TUNEL assays were performed to assess cell viability and apoptosis. BP and BP/LPPC complex delivery efficiency was analyzed by full-wavelength fluorescent scanner and fluorescence microscope. The expres-sions of cell cycle- and apoptosis-related proteins were conducted by Western blotting.ResultsThe results showed that BP inhibited leukemia cell growth by inducing cell cycle arrest and cell apoptosis. LPPC-encapsulated BP rapidly induced endocytic pathway activation, resulting in the internalization of BP into leukemia cells, causing cell apoptosis within 1 h.Conclusions LPPC encapsulation enhanced the cytotoxic activity of BP and did not influence the effects of BP induction that suggested LPPC-encapsulated BP might be developed as anti-leukemia drugs in future.