Effects of various antineoplastic agents on an established human melanoma cell line (G-361)

• García Reverte, Juana María ^[1] ; Bernabeu-Esclapez, A. ^[1] ; Muñoz Ramos, J. ^[1] ; Ortega, Vicente ^[1] ; Canteras Jordana, Manuel ^[1]
  1. [1] Universidad de Murcia

     Universidad de Murcia

     Murcia, España

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 10, Nº. 1, 1995, págs. 79-84
• Idioma: inglés
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• Resumen

  • An established human melanoma cell line was treated with severa1 concentrations of three antineoplastic drugs: melphalan (0.01 6, 0.032, 0.16 pn), CCNU (1- (2-chloroethy1)-3-cyclohexyl1-- nitrosourea) (0.04, 0.21, 0.42 pM) and 4-OHA (4-hydroxyanisole) (4.01 x 1 0-4, 1 . 2 0~1 0 -2~.,4 ~ pM), and the effects on cell growth and viability were compared. 24 hours after treatment, 4-OHA (ID 50=0.016 uM) was more cytotoxic than melphalan (ID50=0.016 pM) and CCNU (ID50=0.21 pM). However. after 96 hours exposure, the most effective drug was CCNU (growth rate = -1.277), which caused the death of the culture. This was followed by melphalan (growth rate = - 1.024) and finally 4-OHA (growth rate= -0.69). Similar ultrastructural cell injuries were observed after the use of the three drugs: the dilation of endoplasmic reticulum vesicles and the nuclear membrane; mitochondria swelling; and the existence of lamellar structures and cytoplasmic vacuoles.